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Journal of Applied Phycology

, Volume 26, Issue 1, pp 607–618 | Cite as

Proteomic profile analysis of Pyropia haitanensis in response to high-temperature stress

  • Yan Xu
  • Changsheng Chen
  • Dehua Ji
  • Nan Hang
  • Chaotian XieEmail author
Article

Abstract

High-temperature stress is a major abiotic stress that affects the yield and quality of Pyropia, which is produced for use as a nutrient. Pyropia haitanensis strain Z-61 is tolerant to high temperature and is widely cultivated in South China. Here, we conducted a comparative proteomic analysis of the blades of Z-61 strain at 29 °C. Changes in the proteome of the blades were analyzed every other day for 8 days at 29 °C. Approximately 1,000 protein species were reproducibly detected at all time points, whereas 87 protein spots were differentially expressed at least at one time point. Fifty-nine protein spots were successfully identified using liquid chromatography–tandem mass spectroscopy and database searching. The proteins were classified into nine functional categories: photosynthesis (27.12 %), energy metabolism (22.03 %), other metabolic pathways (11.86 %), redox homeostasis (11.86 %), response to stimuli (8.47 %), proteins involved in transcription and translation (6.78 %), cytoskeleton-related proteins (5.08 %), proteins that mediate signal transduction (1.69 %), and unknown proteins (5.08 %). These findings indicated that the blades of Z-61 resist high-temperature stress by inhibiting photosynthesis and other nonessential metabolic processes. In contrast, to increase energy metabolism, the expression of proteins related to redox homeostasis and response to stimuli were upregulated, thereby maintaining physiological balance during stress.

Keywords

Pyropia haitanensis Rhodophyta High-temperature stress Proteomic analysis Differentially expressed proteins 

Notes

Acknowledgments

This research was supported in part by the National Natural Science Foundation of China (grant no. 41176151, 41276177), the National High Technology Research & Development Program of China (grant no. 2012AA100811), and the Fund for Distinguished Young Scientists of Fujian Province of China (grant no. 2010J06016).

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Yan Xu
    • 1
  • Changsheng Chen
    • 1
  • Dehua Ji
    • 1
  • Nan Hang
    • 1
  • Chaotian Xie
    • 1
    Email author
  1. 1.College of FisheriesJimei UniversityXiamenPeople’s Republic of China

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