Journal of Applied Phycology

, Volume 25, Issue 5, pp 1435–1439 | Cite as

The effect of high pH on structural lipids in diatoms

  • Kristian Spilling
  • Ása Brynjólfsdóttir
  • Dagmar Enss
  • Heiko Rischer
  • Halldór Guðfinnur Svavarsson
Article

Abstract

We tested the hypothesis that increased pH reduces the amount of structural lipids. To do this, we used three different diatoms (Phaeodactylum tricornutum CCAP strain, P. tricornutum TV strain and Amphiprora sp). We tested the effect of rapid increase from pH 7.5 to 10 by adding NaOH. The total lipid content was reduced by 13, 36 and 47 % in the P. tricornutum CCAP strain, TV strain and Amphiprora sp., respectively, 1 h after increasing the pH. The P. tricornutum CCAP strain was used for further testing the effect of pH on the lipid content during active growth. This strain was cultivated at pH 7.5 and 10, and the pH was regulated by the CO2 inflow. The growth rate was similar (0.3 day−1) in both pH treatments, but the lipid content in the pH 10 treatment was on average 28 % lower than in the pH 7.5 treatment. Our data support the hypothesis that structural lipids are reduced when pH increases to high levels. The results suggest that regulating the pH during algae cultivation could be used to refine the lipid composition in the harvested algal biomass.

Keywords

Membrane lipids Glycolipids Phospholipids Polyunsaturated fatty acids 

Notes

Acknowledgments

This study was funded by the Academy of Finland Research Programme “Sustainable Energy” (SusEn), the Nordic Energy Research Programme “N-INNER” and the Technology Development Fund in Iceland (grant no. 101253011). Additionally, a mobility grant was given to KS from the Nordic Marine Academy. We would also like to thank Hannes Lárus Jóhannsson, Sigrún Helgadóttir, Airi Hyrkäs and Jaana Rikkinen for their excellent technical assistance. The Amphiprora sp was isolated by Sigurbjörn Einarsson.

Supplementary material

10811_2012_9971_MOESM1_ESM.doc (28 kb)
ESM 1(DOC 28 kb)
10811_2012_9971_MOESM2_ESM.doc (27 kb)
ESM 2(DOC 27 kb)

References

  1. Badger MR, Andrews TJ, Whitney SM, Ludwig M, Yellowlees DC, Leggat W, Price GD (1998) The diversity and coevolution of Rubisco, plastids, pyrenoids, and chloroplast-based CO2-concentrating mechanisms in algae. Can J Bot 76:1052–1071Google Scholar
  2. Brown MR, Jeffrey SW, Volkman JK, Dunstan GA (1997) Nutritional properties of microalgae for mariculture. Aquaculture 151:315–331CrossRefGoogle Scholar
  3. Gardner R, Peters P, Peyton B, Cooksey K (2011) Medium pH and nitrate concentration effects on accumulation of triacylglycerol in two members of the Chlorophyta. J Appl Phycol 23:1005–1016CrossRefGoogle Scholar
  4. Guckert J, Cooksey K (1990) Triglyceride accumulation and fatty acid profile changes in Chlorella (Chlorophyta) during high pH-induced cell cycle inhibition. J Phycol 26:72–79CrossRefGoogle Scholar
  5. Guschina I, Harwood J (2006) Lipids and lipid metabolism in eukaryotic algae. Prog Lipid Res 45:160–186PubMedCrossRefGoogle Scholar
  6. Guschina I, Harwood J (2009) Algal lipids and effect of the environment on their biochemistry. In: Arts M, Brett M, Kainz M (eds) Lipids in aquatic ecosystems. Springer, New York, pp 1–24CrossRefGoogle Scholar
  7. Riebesell U, Wolf-Gladrow DA, Smetacek V (1993) Carbon dioxide limitation of marine phytoplankton growth rates. Nature 361:249–251CrossRefGoogle Scholar
  8. Santos AM, Janssen M, Lamers PP, Evers WAC, Wijffels RH (2012) Growth of oil accumulating microalga Neochloris oleoabundans under alkaline-saline conditions. Bioresource Technol 104:593–599CrossRefGoogle Scholar
  9. Smith F, Raven JA (1979) Intracellular pH and its regulation. Ann Rev Plant Physiol 30:289–311CrossRefGoogle Scholar
  10. Williams PJB, Laurens LML (2010) Microalgae as biodiesel & biomass feedstocks: Review & analysis of the biochemistry, energetics & economics. Energ Environ Sci 3:554–590Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Kristian Spilling
    • 1
  • Ása Brynjólfsdóttir
    • 2
  • Dagmar Enss
    • 3
  • Heiko Rischer
    • 3
  • Halldór Guðfinnur Svavarsson
    • 4
  1. 1.Marine Research CentreFinnish Environment InstituteHelsinkiFinland
  2. 2.Blue Lagoon LtdGrindavikIceland
  3. 3.VTT Technical Research Centre of FinlandEspooFinland
  4. 4.School of Science and EngineeringReykjavik UniversityReykjavikIceland

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