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Journal of Applied Phycology

, Volume 25, Issue 5, pp 1405–1412 | Cite as

Inhibitory activities of three Malaysian edible seaweeds on lipase and α-amylase

  • Vimala Balasubramaniam
  • Suraiami Mustar
  • Norhayati Mustafa Khalid
  • Aswir Abd Rashed
  • Mohd Fairulnizal Mohd Noh
  • Matthew D. Wilcox
  • Peter I. Chater
  • Iain A. Brownlee
  • Jeffrey P. Pearson
Article

Abstract

Ethanol extracts, dried powders and fibres (total and soluble fibre) of the tropical red algae Kappaphycus alvarezii, Kappaphycus striatus and Eucheuma denticulatum were analysed for their effect on lipase and α-amylase activity using turbidimetric method and dinitrosalicylic acid (DNS) assay, respectively. The nutrient composition analyses were determined using standard methods. The ethanol extract of dried K. striatus (Ks-III) showed the highest reduction in lipase activity with 92 % inhibition followed by seaweed powders (K. alvarezii (Ka-III), K. striatus (Ks-III) and E. denticulatum (Ed-III)) with average inhibition of 60 %. Soluble fibres of K. alvarezii (Ka-V) and E. denticulatum (Ed-V) showed significant inhibition with 60 and 57 % reduction, respectively. Only the ethanol extract of fresh E. denticulatum (Ed-I) showed 88 % inhibition of α-amylase. Nutritional component analyses showed that all three seaweeds are low in crude fat, suggesting the possible use of seaweed as a dietary supplement and for potential weight and glycaemia management.

Keywords

Nutrient composition Lipase α-Amylase Seaweed Turbidimetric method Dinitrosalicylic acid assay 

Notes

Acknowledgments

The authors thank the Director General of Health Malaysia and the Director of Institute for Medical Research (IMR) for giving the permission to publish this article. We also thank the staff of the Nutrition Unit, Institute for Medical Research for their continuous support. We sincerely thank the Department of Fisheries Sabah, Malaysia for the supply of seaweed samples. This project was funded by the Ministry of Health, Malaysia.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Vimala Balasubramaniam
    • 1
  • Suraiami Mustar
    • 1
  • Norhayati Mustafa Khalid
    • 1
  • Aswir Abd Rashed
    • 1
  • Mohd Fairulnizal Mohd Noh
    • 1
  • Matthew D. Wilcox
    • 2
  • Peter I. Chater
    • 2
  • Iain A. Brownlee
    • 3
  • Jeffrey P. Pearson
    • 2
  1. 1.Cardiovascular, Diabetes and Nutrition Research Centre, Nutrition UnitInstitute for Medical ResearchKuala LumpurMalaysia
  2. 2.Institute for Cell and Molecular Biosciences, The Medical SchoolUniversity of Newcastle Upon TyneNewcastle upon TyneUK
  3. 3.Nanyang Polytechnic Food and Human Nutrition DepartmentNewcastle UniversitySingaporeSingapore

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