Anti-inflammatory activity of phlorotannin-rich fermented Ecklonia cava processing by-product extract in lipopolysaccharide-stimulated RAW 264.7 macrophages
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In this study, fermentation was employed as a tool to further increase the bioactive potential of processing by-product from a brown seaweed, Ecklonia cava, which can be obtained from food and cosmetic industries after its polyphenol extraction. The fermentation process was done for 24 h using an industrially important microorganism Candida utilis prior to being extracted with 80 % ethanol. The anti-inflammatory potential of the fermented E. cava processing by-product extract (FEPBE) was evaluated in vitro. The phlorotannin-rich FEPBE dose-dependently inhibited the nitric oxide production, prostaglandin-E2 production and suppressed the inducible nitric oxide synthase and cyclooxygenase-2 expressions in lipopolysaccharide-stimulated RAW 264.7 cells. The release of pro-inflammatory cytokines, interleukin-1β and interleukin-6, was significantly suppressed by the extract in a dose-dependent manner. Due to the profound anti-inflammatory activity, FEPBE appears as a value-added biomass fraction that can be exploited in numerous industrial applications as a source of functional ingredients.
KeywordsAnti-inflammatory Ecklonia cava Phaeophyta Fermentation Functional ingredients Phlorotannins Macrophages
This research was supported by a grant from the Marine Bioprocess Research Center of the Marine Biotechnology Program funded by the Ministry of Land, Transport, and Maritime, Republic of Korea.
Conflict of interest
The authors declare that they have no conflict of interest.
- Ahmed N, Chen LC, Gordon MA, Laskin JD, Laskin DL (2002) Regulation of cyclooxygenase-2 by nitric oxide in activated hepatic macrophages during acute endotoxemia. J Leukocyte Biol 71:1005–1011Google Scholar
- Chang YC, Li PC, Chen BC, Chang MS, Wang JL, Chiu WT, Lin CH (2006) Lipoteichoic acid-induced nitric oxide synthase expression in RAW 264.7 macrophages is mediated by cyclooxygenase-2, prostaglandin E2, protein kinase A, p38 MAPK, and nuclear factor-κ B pathways. Cell Signal 18:1235–1243PubMedCrossRefGoogle Scholar
- Lee MH, Lee JM, Jun SH, Lee SH, Kim NW, Lee JH, Ko NY, Mun SH, Kim BK, Lim BO, Choi DK, Choi WS (2007) The anti-inflammatory effects of Pyrolae herba extract through the inhibition of the expression of inducible nitric oxide synthase (iNOS) and NO production. J Ethnopharmacol 112:49–54PubMedCrossRefGoogle Scholar
- Park YG, Kang SK, Kim WJ, Lee YC, Kim CH (2004) Effect of TGF-β, IL-β and IL-6 alone or in combination, and tyrosine kinase inhibitor on cyclooxygenase expression, prostaglandin E2 production and bone resorption in mouse calvarial bone cells. Int J Biochem Cell Biol 36:2270–2280PubMedCrossRefGoogle Scholar