Journal of Applied Phycology

, Volume 25, Issue 4, pp 1159–1169 | Cite as

Seasonal variations of total lipids, fatty acid composition, and fucoxanthin contents of Sargassum horneri (Turner) and Cystoseira hakodatensis (Yendo) from the northern seashore of Japan

  • Masatoshi Nomura
  • Hiroyuki Kamogawa
  • Eko Susanto
  • Chikara Kawagoe
  • Hajime Yasui
  • Naotsune Saga
  • Masashi Hosokawa
  • Kazuo MiyashitaEmail author


The present study describes the seasonal changes in lipid components of two brown algae, Sargassum horneri (Turner) and Cystoseira hakodatensis (Yendo), with specific reference to fucoxanthin (Fx) and n-3 (omega-3) polyunsaturated fatty acids (PUFAs). Young thalli of S. horneri were collected from different places, Nesaki and Matsushima, and cultivated in the same place. Total lipids (TL) and Fx contents of S. horneri increased from October or November and reached a maximum in January and then decreased thereafter. TL and Fx of S. horneri originated from Matsushima were always higher than those originated from Nesaki, suggesting that genetic variation might be a reason for the different TL and Fx contents found in both S. horneri seeds from different origin. Similar seasonal variation in the TL and Fx content has been more clearly observed in C. hakodatensis. Maximum TL content of S. horneri and C. hakodatensis reached to more than 14 and 15 % in winter season. These values were much higher than those found in previous studies showing TL content range from 1 to 5 %. Fatty acid composition of lipids from S. horneri and C. hakodatensis also changed seasonally. Total monounsaturated fatty acids showed the lower values from January to April, while there were no big variations in total saturated fatty acids and total n-6 PUFAs. On the other hand, total n-3 PUFAs became highest in winter. Overall, S. horneri and C. hakodatensis harvested from winter to spring will be used as good n-3 PUFA source with high levels of TL and Fx.


Brown algae Fatty acids Omega-3 PUFA Fucoxanthin 


  1. Airanthi MKWA, Sasaki N, Iwasaki S, Baba N, Abe M, Hosokawa M, Miyashita K (2011) Effect of brown seaweed lipids on fatty acid composition and lipid hydroperoxide levels of mouse liver. J Agric Food Chem 59:4156–4163PubMedCrossRefGoogle Scholar
  2. Allayee H, Roth N, Hodis HN (2009) Polyunsaturated fatty acids and cardiovascular disease: implications for nutrigenetics. J Nutrigenet Nutrigenomics 2:140–148PubMedCrossRefGoogle Scholar
  3. Arao T, Yamada M (1989) Positional distribution of fatty acids in galactolipids of algae. Phytochemistry 28:805–810CrossRefGoogle Scholar
  4. Bhaskar N, Miyashita K (2005) Lipid composition of Padina tetrastomatica (Dictyotalea, Phaeophyta), brown seaweed of the west coast of India. Indian J Fish 52:263–268Google Scholar
  5. Bhaskar N, Hosokawa M, Miyashita K (2004) Comparitive evaluation of fatty acid composition of different Sargassum (Fucales, Phaeophyta) species harvested from temperate and tropical waters. J Aquat Product Technol 13:53–70CrossRefGoogle Scholar
  6. Cohen Z, Vonshak A, Richmond A (1998) Effect of environmental conditions on fatty acid composition of the red alga Porphyridium cruentum: correlation to growth rate. J Phycol 24:328–332Google Scholar
  7. Dembitsky VM, Rozentsvet OA, Pechenkina EE (1990) Glycolipids, phospholipids and fatty acids of brown algae species. Phytochemistry 29:3417–3421CrossRefGoogle Scholar
  8. Eichenberger W, Araki S, Moller D (1993) Betaine lipids and phospholipids in brown algae. Phytochemistry 34:1323–1333CrossRefGoogle Scholar
  9. Eonseon J, Polle JEW, Lee HK, Hyun SM, Chang M (2003) Xanthophylls in microalgae: from biosynthesis to biotechnological mass production and application. J Microbiol Biotechnol 13:165–174Google Scholar
  10. Gerasimenko NI, Skriptsova AV, Moiseenko OP (2010) Seasonal changes in the content of lipids, fatty acids, and pigments in brown alga Costaria costata. Russ J Plant Physiol 57:205–211CrossRefGoogle Scholar
  11. Gerasimenko NI, Skriptsova AV, Busarova NG, Moiseenko OP (2011) Effects of the season and growth stage on the contents of lipids and photosynthetic pigments in brown alga Undaria pinnatifida. Russ J Plant Physiol 58:885–891CrossRefGoogle Scholar
  12. Givens DI, Gibbs RA (2008) Current intakes of EPA and DHA in European populations and the potential of animal-derived foods to increase them. Proc Nutr Soc 67:273–280CrossRefGoogle Scholar
  13. Harris WS, Miller M, Tighe AP, Davidson MH, Schaefer EJ (2008) Omega-3 fatty acids and coronary heart disease risk: clinical and mechanistic perspectives. Atherosclerosis 197:12–24PubMedCrossRefGoogle Scholar
  14. Hoffman DR, Boettcher JA, Diersen-Schade DA (2009) Toward optimizing vision and cognition in term infants by dietary docosahexaenoic and arachidonic acid supplementation: a review of randomized controlled trials. Prostaglandins Leukot Essent Fatty Acids 81:151–158PubMedCrossRefGoogle Scholar
  15. Honya M, Kinoshita T, Ishikawa M, Mori H, Nisizawa K (1994) Seasonal variation in the lipid content of cultured Laminaria japonica: fatty acids, sterols, β-carotene and tocopherol. J Appl Phycol 6:25–29CrossRefGoogle Scholar
  16. Hosokawa M, Miyashita T, Nishikawa S, Emi S, Tsukui T, Beppu F, Okada T, Miyashita K (2010) Fucoxanthin regulates adipocytokine mRNA expression in white adipose tissue of diabetic/obese KK-A y mice. Arch Biochem Biophys 504:17–25PubMedCrossRefGoogle Scholar
  17. Hu Z-M, Uwai S, Yu S-H, Komatsu T, Ajisaka T, Duan D-L (2011) Phylogeographic heterogeneity of the brown macroalga Sargassum horneri (Fucaceae) in the northwestern Pacific in relation to late Pleistocene glaciation and tectonic configurations. Mol Ecol 20:3894–3909PubMedCrossRefGoogle Scholar
  18. Ishihara K, Murata M, Kaneniwa M, Saito H, Komatsu W, Shinohara K (2000) Purification of stearidonic acid (18:4(n-3)) and hexadecatetraenoic acid (16:4(n-3)) from algal fatty acid with lipase and medium pressure liquid chromatography. Biosci Biotechnol Biochem 64:2454–2457PubMedCrossRefGoogle Scholar
  19. Jones AL, Harwood JL (1992) Lipid composition of the brown algae Fucus serratus and Ascophyllum nodosum. Phytochemistry 31:3397–3403CrossRefGoogle Scholar
  20. Khan MNA, Cho J-Y, Lee M-C, Kang J-Y, Park NG, Fuji H, Hong Y-K (2007) Isolation of two anti-inflammatory and one pro-inflammatory polyunsaturated fatty acids from the brown seaweed Undaria pinnatifida. J Agric Food Chem 55:6984–6988PubMedCrossRefGoogle Scholar
  21. Khotimchenko SV (1998) Fatty acids of brown algae from the Russian Far East. Phytochemistry 49:2363–2369CrossRefGoogle Scholar
  22. Kim M-K, Dubacq J-P, Thomas J-C, Giraud G (1996) Seasonal variations of triacylglycerols and fatty acids in Fucus serratus. Phytochemistry 43:49–55CrossRefGoogle Scholar
  23. Le HD, Meisel JA, de Meijer VE, Gura KM, Puder M (2009) The essentiality of arachidonic acid and docosahexaenoic acid. Prostaglandins Leukot Essent Fatty Acids 81:165–170PubMedCrossRefGoogle Scholar
  24. Leaf A, Kang JX, Xiao Y-F (2008) Fish oil fatty acids as cardiovascular drugs. Curr Vasc Pharmacol 6:1–12PubMedCrossRefGoogle Scholar
  25. Li X, Fan X, Han L, Lou Q (2002) Fatty acids of some algae from the Bohai Sea. Phytochemistry 59:157–161PubMedCrossRefGoogle Scholar
  26. Maeda H, Hosokawa M, Sashima T, Funayama K, Myashita K (2005) Fucoxanthin from edible seaweed, Undaria pinnatifida, shows antiobesity effect through UCP1 expression in white adipose tissues. Biochem Biophys Res Commun 332:392–397PubMedCrossRefGoogle Scholar
  27. Miyashita K, Nishikawa S, Beppu F, Tsukui T, Abe M, Hosokawa M (2011) Allenic carotenoid, fucoxanthin, as a novel marine nutraceutical from brown seaweed. J Sci Food Agric 91:1166–1174PubMedCrossRefGoogle Scholar
  28. Mouget J-L, Rosa P, Tremblin G (2004) Acclimation of Haslea ostrearia to light of different spectral qualities—confirmation of ‘chromatic adaptation’ in diatoms. J Photochem Photobiol B 75:1–11PubMedCrossRefGoogle Scholar
  29. Narayan B, Hosokawa M, Miyashita K (2004a) Comparative evaluation of fatty acid composition of different Sargassum (Fucales, Phaeophyta) species harvested from temperate and tropical waters. J Aquat Product Technol 13:53–70CrossRefGoogle Scholar
  30. Narayan B, Hosokawa M, Miyashita K (2004b) Growth inhibition of human pro-myelocytic leukemia (9HL-60) cells by lipid extracts of marine alga Sargassam marginatum (Fucales, Phaeophyta) harvested off Goa (west coast of India) with special reference to fatty acid composition. Indian J Mar Sci 33:355–360Google Scholar
  31. Narayan B, Tomohisa K, Miyashita K, Park S-B, Endo Y, Fujimoto K (2004c) Occurrence of conjugated polyenoic fatty acids in seaweeds from the Indian Ocean. Z Naturforsch 59c:310–314Google Scholar
  32. Nelson MM, Phleger CF, Nichols PD (2002) Seasonal lipid composition in macroalgae of the northeastern Pacific Ocean. Bot Mar 45:58–65CrossRefGoogle Scholar
  33. Nishikawa S, Hosokawa M, Miyashita K (2012) Fucoxanthin promotes translocation and induction of glucose transporter 4 in skeletal muscles of diabetic/obese KK-A y mice. Phytomedicine 19:389–394PubMedCrossRefGoogle Scholar
  34. Prevot AF, Mordret FX (1976) Utilisation des colonnes capillaires de verre pour l’analyse des corps gras par chromotographie en phase gazeuse. Rev Fr Corps Gras 23:409–423Google Scholar
  35. Russo GL (2010) Dietary n-6 and n-3 polyunsaturated fatty acids: from biochemistry to clinical implications in cardiovascular prevention. Biochem Pharmacol 235:785–795Google Scholar
  36. Sánchez-Machado DI, López-Cervantes J, López-Hernández J, Paseiro-Losada P (2004a) Fatty acids, total lipid, protein and ash contents of processed edible seaweeds. Food Chem 85:439–444CrossRefGoogle Scholar
  37. Sánchez-Machado DI, López-Hernández P, Paseiro-Losada P, López-Cervantes J (2004b) An HPLC method for the quantification of sterols in edible seaweeds. Biomed Chromatogr 18:183–190PubMedCrossRefGoogle Scholar
  38. Sanina NM, Goncharova SN, Kostetsky EY (2008) Seasonal changes of fatty acid composition and thermotropic behavior of polar lipids from marine macrophytes. Phytochemistry 69:1517–1527PubMedCrossRefGoogle Scholar
  39. Sewon P, Mikola H, Lehtinen T, Kallio P (1997) Polar lipids and net photosynthesis potential of subarctic Diapensia lapponica. Phytochemistry 46:1339–1347CrossRefGoogle Scholar
  40. Smith KL, Harwood JL (1984) Lipids and lipid metabolism in the brown alga, Fucus serratus. Phytochemistry 23:2469–2473CrossRefGoogle Scholar
  41. Southichak B, Nakano K, Nomura M, Chiba N, Nishimura O (2008) Marine macroalga Sargassum horneri as biosorbent for heavy metal removal: roles of calcium in ion exchange mechanism. Water Sci Technol 58:697–704PubMedCrossRefGoogle Scholar
  42. Terasaki M, Baba Y, Yasui H, Saga N, Hosokawa M, Miyashita K (2009) Evaluation of recoverable functional lipid components with special reference to fucoxanthin and fucosterol contents of several brown seaweeds of Japan. J Phycol 45:974–980CrossRefGoogle Scholar
  43. Tziomalos K, Athyros VG, Karagiannis A, Mikhailidis DP (2008) Omega-3 fatty acids: how can they be used in secondary prevention? Curr Atheroscler Rep 10:510–517PubMedCrossRefGoogle Scholar
  44. Van Ginneken VJT, Helsper JPFG, de Visser W, van Keulen H, Brandenburg WA (2011) Polyunsaturated fatty acids in various macroalgal species from north Atlantic and tropical seas. Lipids Health Dis 10:104PubMedCrossRefGoogle Scholar
  45. Vaskovsky VE, Khotimchenko SV, Xia B, Hefang L (1996) Polar lipids and fatty acids of some marine macrophytes from the Yellow Sea. Phytochemistry 42:1347–1356CrossRefGoogle Scholar
  46. Worm B, Hilborn R, Baum JK, Branch TA, Collie JS, Costello C, Fogarty MJ, Fulton EA, Hutchings JA, Jennings S, Jensen OP, Lotze HK, Mace PM, McClanahan TR, Minto C, Palumbi SR, Parma AM, Ricard D, Rosenberg AA, Watson R, Zeller D (2009) Rebuilding global fisheries. Science 325:578–585PubMedCrossRefGoogle Scholar
  47. Zhukova NV, Titlyanov EA (2006) Effect of light intensity on the fatty acid composition of dinoflagellates symbiotic with hermatypic corals. Bot Mar 49:339–346CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Masatoshi Nomura
    • 1
  • Hiroyuki Kamogawa
    • 1
  • Eko Susanto
    • 1
  • Chikara Kawagoe
    • 2
  • Hajime Yasui
    • 1
  • Naotsune Saga
    • 1
  • Masashi Hosokawa
    • 1
  • Kazuo Miyashita
    • 1
    Email author
  1. 1.Faculty of Fisheries SciencesHokkaido UniversityHakodateJapan
  2. 2.Algatech KyowaKyowa Concrete Industry Co. Ltd.HakodateJapan

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