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Journal of Applied Phycology

, Volume 25, Issue 4, pp 1017–1021 | Cite as

Effects of carbon sources on growth and extracellular polysaccharide production of Nostoc flagelliforme under heterotrophic high-cell-density fed-batch cultures

  • Zhen Ding
  • Shiru Jia
  • Peipei Han
  • Nannan Yuan
  • Ning Tan
Article

Abstract

Dissociated cells separated from a natural colony of Nostoc flagelliforme were cultivated heterotrophically in the darkness on glucose under fed-batch culture conditions. The effects of carbon sources (glucose, fructose, xylose, and sucrose) and concentrations on cell growth and extracellular polysaccharide (EPS) production were investigated. At harvest, the culture contained 1.325 g L−1 of biomass and 117.2 mg L−1 of EPS, respectively. The gravimetric EPS production rate was 16.7 mg g−1 cell dry weight day−1, which was 2.1 times higher than previously reported. Using sigmoid model, batch fermentation of N. flagelliforme was kinetically simulated to obtain equations including substrate consumption, biomass growth, and EPS accumulation. Results from a simulation correlated well with the experimental ones, indicating that this method could be useful in studying EPS production from batch and fed-batch cultures.

Keywords

Nostoc flagelliforme Cyanobacteria Heterotrophic Fed-batch culture Extracellular polysaccharides (EPS) 

Notes

Acknowledgments

This work is supported by a grant from the National Natural Science Foundation of China, no. 31201405.

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Zhen Ding
    • 1
    • 2
  • Shiru Jia
    • 1
  • Peipei Han
    • 1
  • Nannan Yuan
    • 1
  • Ning Tan
    • 1
  1. 1.Key Laboratory of Industrial Microbiology, Ministry of EducationTianjin University of Science and TechnologyTianjinChina
  2. 2.Department of Food EngineeringRizhao Polytechnic CollegeRizhaoChina

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