Journal of Applied Phycology

, Volume 24, Issue 6, pp 1503–1507 | Cite as

Estimating bioavailability of soil particulate phosphorus to Microcystis aeruginosa

  • Yoko OkuboEmail author
  • Takanobu Inoue
  • Kuriko Yokota


Bioavailable phosphorus has been reported to include not only phosphorus in dissolved form but also the fraction of particulate phosphorus that is readily usable. Here, a chemical extraction scheme in combination with an algal bioassay was conducted to estimate the potential bioavailability of particulate phosphorus in soil collected from a Chinese cabbage field. Microcystis aeruginosa was cultured in a medium containing the soils sequentially extracted with 1 M NH4Cl, 0.11 M bicarbonate dithionite, 1 M NaOH, and 0.5 M HCl as the sole source of phosphorus. Analyses of chlorophyll-a, suspended solids, particulate organic carbon, and particulate organic nitrogen showed that M. aeruginosa could utilize some of the phosphorus present in each soil extract. These results imply that bioavailable phosphorus includes not only aluminum/iron-bound P and clay-bound P but also carbonate/apatite-bound P and residual P which have been considered not to be available to algae.


Particulate phosphorus Bioavailable phosphorus Microcystis aeruginosa Cyanobacteria Sequential phosphorus extraction 



This research was financially supported by the Environment Research and Technology Development Fund (B-0908) of the Ministry of the Environment, Japan.


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© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  1. 1.Department of Architecture and Civil Engineering, Graduate School of EngineeringToyohashi University of TechnologyToyohashiJapan

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