Journal of Applied Phycology

, Volume 24, Issue 4, pp 715–723

Designed optimization of a single-step extraction of fucose-containing sulfated polysaccharides from Sargassum sp.

  • Marcel Tutor Ale
  • Jørn Dalgaard Mikkelsen
  • Anne S. Meyer
Article

Abstract

Fucose-containing sulfated polysaccharides can be extracted from the brown seaweed, Sargassum sp. It has been reported that fucose-rich sulfated polysaccharides from brown seaweeds exert different beneficial biological activities including anti-inflammatory, anticoagulant, and anti-viral effects. Classical extraction of fucose-containing sulfated polysaccharides from brown seaweed species typically involves extended, multiple-step, hot acid, or CaCl2 treatments, each step lasting several hours. In this work, we systematically examined the influence of acid concentration (HCl), time, and temperature on the yield of fucose-containing sulfated polysaccharides (FCSPs) in statistically designed two-step and single-step multifactorial extraction experiments. All extraction factors had significant effects on the fucose-containing sulfated polysaccharides yield, with the temperature and time exerting positive effects, and the acid concentration having a negative effect. The model defined an optimized single-step FCSPs extraction procedure for Sargassum sp. (a brown seaweed). A maximal fucose-containing sulfated polysaccharides yield of ∼7% of the Sargassum sp. dry matter was achieved by the optimal extraction procedure of: 0.03 M HCl, 90°C, 4 h. HPAEC-PAD analysis confirmed that fucose, galactose, and glucuronic acid were the major constituents of the polysaccharides obtained by the optimized method. Lower polysaccharide yield, but relatively higher fucose content was obtained with shorter extraction time. The data also revealed that classical multi-step extraction with acid ≥0.2 M HCl at elevated temperature and extended time had a detrimental effect on the FCSPs yield as this treatment apparently disrupted the structural integrity of the polymer and evidently caused degradation of the carbohydrate chains built up of fucose residues.

Keywords

Fucoidan Sargassum Brown seaweed Fucose Bioactive compound Extraction method 

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Marcel Tutor Ale
    • 1
  • Jørn Dalgaard Mikkelsen
    • 1
  • Anne S. Meyer
    • 1
  1. 1.Center for Bioprocess Engineering, Department of Chemical and Biochemical EngineeringTechnical University of Denmark (DTU)Kgs. LyngbyDenmark

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