Journal of Applied Phycology

, Volume 23, Issue 4, pp 745–753 | Cite as

Purification and characterization of a lectin, BPL-3, from the marine green alga Bryopsis plumosa

  • Jong Won Han
  • Kang Sup Yoon
  • Tatyana A. Klochkova
  • Mi-Sook Hwang
  • Gwang Hoon Kim
Article

Abstract

A novel lectin was isolated and characterized from Bryopsis plumosa (Hudson) Agardh and named BPL-3. This lectin showed specificity to N-acetyl-d-galactosamine as well as N-acetyl-d-glucosamine and agglutinated human erythrocytes of all blood types, showing slight preference to the type A. SDS-PAGE and MALDI-TOF MS data showed that BPL-3 was a monomeric protein with molecular weight of 11.5 kDa. BPL-3 was a non-glycoprotein with pI value of ∼7.0. It was stable in high temperatures up to 70°C and exhibited optimum activity in pH 5.5–10. The N-terminal and internal amino acid sequences of the lectin were determined by Edman degradation and enzymatic digestion, which showed no sequence homology to any other reported proteins. The full sequence of the cDNA encoding this lectin was obtained from PCR using cDNA library, and the degenerate primers were designed from the N-terminal amino acid sequence. The size of the cDNA was 622 bp containing single ORF encoding the lectin precursor. This lectin showed the same sugar specificity to previously reported lectin, Bryohealin, involved in protoplast regeneration of B. plumosa. However, the amino acid sequences of the two lectins were completely different. The homology analysis of the full cDNA sequence of BPL-3 showed that it might belong to H lectin group, which was originally isolated from Roman snails.

Keywords

BPL-3 N-acetyl-d-glucosamine N-acetyl-d-galactosamine Bryohealin Bryopsis plumosa Lectin Protoplast regeneration 

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Jong Won Han
    • 1
  • Kang Sup Yoon
    • 1
  • Tatyana A. Klochkova
    • 1
  • Mi-Sook Hwang
    • 2
  • Gwang Hoon Kim
    • 1
  1. 1.Department of BiologyKongju National UniversityKongjuSouth Korea
  2. 2.Seaweed Research CenterNational Fisheries Research and Development InstituteMokpoSouth Korea

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