Chemopreventive effect of Padina boergesenii extracts on ferric nitrilotriacetate (Fe-NTA)-induced oxidative damage in Wistar rats
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The present study was designed to investigate the prophylactic effect of extracts of the brown alga Padina boergesenii against potent nephrotoxic agent ferric nitrilotriacetate (Fe-NTA), in blood circulation of rats. Administration of Fe-NTA for seven consecutive days significantly enhanced lipid peroxidation accompanied with reduction in glutathione content. Together with this, the level of antioxidant enzymes, glutathione peroxidase, superoxide dismutase, and catalase was significantly (P < 0.05) diminished. Pretreatment of rats with P. boergesenii (150 mg kg−1 body weight) reversed Fe-NTA-induced oxidative damage in lipid peroxidation and glutathione content significantly (P < 0.05). Further, the activity of antioxidant enzymes was also restored significantly. In order to assess the role of polyphenolic components in the relevant activity, phenolic contents of the extract was found to be 1.78 ± 0.02% in the methanol extract and 1.30 ± 0.30% in the diethyl ether extract. Hence, the present results confirm that the brown alga P. boergesenii preclude its role in Fe-NTA-induced oxidative damage and hyperproliferative response in circulation.
KeywordsFerric nitrilotriacetate Oxidative stress Total phenolics Antioxidant activity
We are grateful to Dr. P. Subramanian, Reader, Department of Biochemistry and Biotechnology, Annamalai University for valuable support in technical aspects. The authors wish to thank Department of Biotechnology (DBT), India for the financial support. Finally, the authors would like to acknowledge the three anonymous referees for their constructive criticism and their help to clarify and improve this manuscript.
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