Journal of Applied Phycology

, Volume 23, Issue 1, pp 35–45 | Cite as

An AFLP-based survey of genetic diversity and relationships of major farmed cultivars and geographically isolated wild populations of Saccharina japonica (Phaeophyta) along the northwest coasts of the Pacific

  • Ti Feng Shan
  • Shao Jun PangEmail author
  • Yu Rong Zhang
  • Irina M. Yakovleva
  • Anna V. Skriptsova


The genetic diversity and relationships of six representative cultivars and six geographically isolated wild populations of Saccharina japonica along the northwest coasts of the Pacific Ocean were investigated using AFLP markers. A total of 547 bands were generated across all samples by ten primer combinations. At the cultivar or population level, the percentage of polymorphic loci (P), gene diversity (H), and Shannon’s information index (I) was highest in Dalian population (P 59.05%; H 0.2057; I 0.3062) and lowest in Lianjiang cultivar (P 9.87%; H 0.0331; I 0.0497). At the species level, P, H, and I were 85.01%, 0.1948, and 0.3096, respectively. Unique bands were detected in all the six wild populations, with Dalian being the most. In comparison, only Yanza cultivar possessed one unique band. The G ST value was 0.6226 and the gene flow (N m ) was 0.1515, indicating strong genetic differentiation among cultivars and populations. Two UMPGA dendrograms were constructed based on the Dice similarity coefficients among individuals and on genetic distances among cultivars and populations, which generally revealed three major clades corresponding to three countries. Analysis of molecular variance revealed that a larger proportion (60.21%) of the total genetic variation was attributable to differences among cultivars and populations. The Mantel test suggested that genetic differentiation was positively correlated with geographic distance (r = 0.7962, P = 0.011) in the six wild populations, agreeing with the isolation by distance model. On the whole, low to moderate genetic diversity within cultivars and populations (except Dalian population) and high genetic differentiation among cultivars and populations were detected.


AFLP Cultivar Genetic differentiation Genetic diversity Population structure Saccharina japonica 



The authors wish to thank Hyung Geun Kim and Kyu Sam Han from Kangnung-Wonju National University for collecting the algal samples, Su Qin Gao, Shi Guo Li, Chun Hua Song, and Jing Pu Zhang for the great help in sample handling and some of the experimental work. Special thanks go to the anonymous reviewers for their invaluable suggestions and comments. This investigation was financially supported by the following programs: (1) the 863 Hi-Tech Research and Development Program of China (2006AA10A412;2006AA10A416); (2) a project from the Chinese Academy of Sciences (KSCX2-YW-N-47-07); (3) a project from the Ministry of Science and technology of China (2006DKA30470-017); (4) a China–Russia bilateral cooperation program between the Chinese Academy of Sciences and the Far East Branch of Russian Academy of Sciences (program no. 2008-21#); (5) a project from Ministry of Agriculture of China (200903030).


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Ti Feng Shan
    • 1
  • Shao Jun Pang
    • 1
    Email author
  • Yu Rong Zhang
    • 2
  • Irina M. Yakovleva
    • 3
  • Anna V. Skriptsova
    • 3
  1. 1.Marine Biological Culture Collection Center, Institute of OceanologyChinese Academy of SciencesQingdaoChina
  2. 2.Fisheries Research Institute of Zhejiang ProvinceZhoushanChina
  3. 3.Institute of Marine BiologyFar Eastern Branch of Russian Academy of SciencesVladivostokRussia

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