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Journal of Applied Phycology

, Volume 23, Issue 1, pp 1–6 | Cite as

Single-step chromatography for simultaneous purification of C-phycocyanin and allophycocyanin with high purity and recovery from Spirulina (Arthrospira) platensis

  • Shi-Gan Yan
  • Li-Ping Zhu
  • Hai-Nan Su
  • Xi-Ying Zhang
  • Xiu-Lan Chen
  • Bai-Cheng Zhou
  • Yu-Zhong ZhangEmail author
Article

Abstract

The cyanobacterium Spirulina (Arthrospira) platensis is a good source of phycobiliprotein purification. C-phycocyanin (C-PC) is the major phycobiliprotein, while allophycocyanin (APC) is less abundant in S. platensis. Previously reported methods for C-PC purification are only able to offer either high purity or high efficiency. This paper describes one-step anion exchange chromatography method with continuous pH gradient elution for simultaneous purification of C-PC and APC with high purity and high recovery. Crude C-PC and APC were extracted and concentrated by ammonium sulfate fractionation at saturation of 25% and 60%, then purified on a DEAE-Sepharose Fast Flow chromatography column with continuous pH gradient elution from pH 5.0 to 3.6. After this single-step chromatography, C-PC and APC with high purity and recovery were simultaneously obtained. The purity ratios of C-PC and APC reached 5.59 (A620/A280) and 5.19 (A650/A280), respectively. Their purity was further demonstrated by electrophoresis and fluorescence emission spectroscopy. Moreover, the total recovery yield of pure C-PC and APC were 67.04% and 80.0%, representing 111.83 and 29.28 mg·g−1 lyophilized weight, respectively. The obtained C-PC and APC remained stable over a pH range of 4–9. This purification method for high purity and recovery of C-PC and APC proved to be fairly efficient compared with previously reported methods.

Keywords

C-phycocyanin Allophycocyanin Anion exchange chromatography pH gradient Purification 

Notes

Acknowledgments

The work was supported by the Hi-Tech Research and Development program of China (2008AA09Z404), the National Natural Science Foundation of China (40676078), and the Scientific and Technological Development Program of Shandong Province (2006GG2205009).

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Shi-Gan Yan
    • 1
    • 3
  • Li-Ping Zhu
    • 2
  • Hai-Nan Su
    • 1
  • Xi-Ying Zhang
    • 1
  • Xiu-Lan Chen
    • 1
  • Bai-Cheng Zhou
    • 1
  • Yu-Zhong Zhang
    • 1
    Email author
  1. 1.State Key Lab of Microbial TechnologyShandong UniversityJinanChina
  2. 2.School of Food and BioengineeringShandong Institute of Light IndustryJinanChina
  3. 3.Shandong Academy of Agricultural SciencesJinanChina

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