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Journal of Applied Phycology

, Volume 21, Issue 5, pp 493–507 | Cite as

Lipid productivity as a key characteristic for choosing algal species for biodiesel production

  • Melinda J. Griffiths
  • Susan T. L. Harrison
Article

Abstract

Microalgae are a promising alternative source of lipid for biodiesel production. One of the most important decisions is the choice of species to use. High lipid productivity is a key desirable characteristic of a species for biodiesel production. This paper reviews information available in the literature on microalgal growth rates, lipid content and lipid productivities for 55 species of microalgae, including 17 Chlorophyta, 11 Bacillariophyta and five Cyanobacteria as well as other taxa. The data available in the literature are far from complete and rigorous comparison across experiments carried out under different conditions is not possible. However, the collated information provides a framework for decision-making and a starting point for further investigation of species selection. Shortcomings in the current dataset are highlighted. The importance of lipid productivity as a selection parameter over lipid content and growth rate individually is demonstrated.

Keywords

Algal biodiesel Lipid productivity Species selection 

Notes

Acknowledgements

This work is based upon research supported by the South African Research Chair Initiative of the Department of Science and Technology and the National Research Foundation. The financial assistance of the National Research Foundation (NRF) towards this research is hereby acknowledged. Opinions expressed and conclusions arrived at are those of the authors and are not necessarily to be attributed to the NRF.

References

  1. Adam MS (1997) Metabolic response of the halotolerant green alga Dunaliella bardawil to nitrogen:phosphorous ratios in batch culture. Folia Microbiol 42:357–360CrossRefGoogle Scholar
  2. Ahmad I, Hellebust A (1990) Regulation of chloroplast development by nitrogen source and growth conditions in a Chlorella protothecoides strain. Plant Physiol 94:944–949PubMedCrossRefGoogle Scholar
  3. Apt KE, Behrens PW (1999) Commercial developments in microalgal biotechnology (review). J Phycol 35:215–226CrossRefGoogle Scholar
  4. Baker JW, Grover JP, Brroks BW, Urena-Boeck F, Roelke DL, Errera R, Kiesling RL (2007) Growth and toxicity of Prymnesium parvum (Haptophyta) as a function of salinity, light, and temperature. J Phycol 43(2):219–227CrossRefGoogle Scholar
  5. Banerjee A, Sharma R, Chisti Y, Banerjee UC (2002) Botryococcus braunii: a renewable source of hydrocarbons and other chemicals. Crit Rev Biotechnol 22(3):245–279PubMedCrossRefGoogle Scholar
  6. Becker EW (1994) Microalgae: biotechnology and microbiology. Cambridge University Press, Cambridge, UKGoogle Scholar
  7. Ben-Amotz A, Tornabene TG (1985) Chemical profile of selected species of microalgae with emphasis on lipids. J Phycol 21:72–81Google Scholar
  8. Benemann JR, Weissman JC, Koopman BL, Oswald WJ (1977) Energy production by microbial photosynthesis. Nature 268:19–23CrossRefGoogle Scholar
  9. Benider A, Tahiri M, Belkoura M, Dauta A (2001) Interacting effect of heliothermic factors on the growth rate of 3 Scenedesmus species. Int J Lim 37:257–266CrossRefGoogle Scholar
  10. Beudeker RF, Tabita FR (1983) Control of photorespiration gycolate metabolism in an oxygen-resistant mutant of Chlorella sorokiniana. J Bacteriol 155:650–656PubMedGoogle Scholar
  11. Bhaud Y, Salmon J-M, Soyer-Gobillard M-O (1991) The complex cell cycle of the dinoflagellate protoctist Crypthecodinium cohnii as studied in vivo and by cytofluorimetry. J Cell Sci 100:675–682Google Scholar
  12. Bopp SK, Lettieri T (2007) Gene regulation in the marine diatom Thalassiosira pseudonana upon exposure to polycyclic aromatic hydrocarbons (PAHs). Gene 396:293–302PubMedCrossRefGoogle Scholar
  13. Borowitzka MA (1992) Algal biotechnology products and processes—matching science and economics. J Appl Phycol. 4:267–279CrossRefGoogle Scholar
  14. Borowitzka MA (1997) Microalgae for aquaculture: opportunities and constraints. J Appl Phycol 9:393–401CrossRefGoogle Scholar
  15. Burlew JS (1953) Algal culture, from laboratory to pilot plant. Carnegie Institute of Washington, Washington D.CGoogle Scholar
  16. Butterwick C, Heaney SI, Talling JF (2005) Diversity in the influence of temperature on the growth rates of freshwater algae, and its ecological relevance. Freshw Biol 50:291–300Google Scholar
  17. Ceron Garcia MC, Fernandez Sevilla JM, Acien Fernandez FG, Molina Grima E, Garcia Camacho F (2000) Mixotrophic growth of Phaeodactylum tricornutum on glycerol: growth rate and fatty acid profile. J Appl Phycol 12:239–248CrossRefGoogle Scholar
  18. Chelf P (1990) Environmental control of lipid and biomass production in two diatom species. J Appl Phycol 2:121–129CrossRefGoogle Scholar
  19. Chen Y-C (2007) Immobilization of twelve benthic diatom species for long-term storage and as feed for post-larval abalone Haliotis diversicolor. Aquaculture 263(1–4):97–106CrossRefGoogle Scholar
  20. Chisti Y (2007) Biodiesel from microalgae. Biotechnol Adv 25:294–306PubMedCrossRefGoogle Scholar
  21. Chisti Y (2008) Biodiesel from microalgae beats bioethanol. Trends Biotechnol 26:126–131PubMedCrossRefGoogle Scholar
  22. Coleman LW, Rosen BH, Schwartzbach SD (1988) Environmental control of carbohydrate and lipid synthesis in Euglena. Plant Cell Physiol 29:423–432Google Scholar
  23. Collyer DM, Fogg GE (1954) Studies on fat accumulation by algae. J Exp Bot 6:256–275CrossRefGoogle Scholar
  24. Constantopoulos G, Bloch K (1967) Effect of light intensity on the lipid composition of Euglena gracilis. J Biol Chem 242:3538–3542Google Scholar
  25. Cook JR (1966) Adaptations to temperature in two closely related strains of Euglena gracilis. Biol Bull 131:83–93CrossRefGoogle Scholar
  26. Coombs J, Darley WM, Holm-Hansen O, Volcani BE (1967) Studies on the biochemistry and fine structure of silica shell formation in diatoms. Chemical composition of Navicula pelliculosa during silicon-starvation synchrony. Plant Physiol 42:1601–1606PubMedCrossRefGoogle Scholar
  27. De la Pena MR (2007) Cell growth and nutritive value of the tropical benthic diatom, Amphora sp., at varying levels of nutrients and light intensity, and different culture locations. J Appl Phycol 19:647–655CrossRefGoogle Scholar
  28. Dempster TA, Sommerfeld MR (1998) Effects of environmental conditions on growth and lipid accumulation in Nitzschia communis (Bacillariophyceae). J Phycol 34:712–721CrossRefGoogle Scholar
  29. Exley C, Tollervey A, Gray G, Roberts S, Birchall JD (1993) Silicon, aluminium and the biological availability of phosphorous in algae. Proc R Soc Lond B 253(1336):93–99CrossRefGoogle Scholar
  30. Ferguson RL, Collier A, Meeter DA (1976) Growth response of Thalassiosira pseudonana Hasle and Hemdal clone 3H to illumination, temperature and nitrogen source. Chesap Sci 17(3):148–158CrossRefGoogle Scholar
  31. Fisher T, Minnaard J, Dubinsky Z (1996) Photoacclimation in the marine alga Nannochloropsis sp. (Eustigmatophyte): a kinetic study. J Plankton Res 18:1797–1818CrossRefGoogle Scholar
  32. Gatenby CM, Orcutt DM, Kreeger DA, Parker BC, Jones VA, Neves RJ (2003) Biochemical composition of three algal species proposed as food for captive freshwater mussels. J Appl Phycol 15:1–11CrossRefGoogle Scholar
  33. Goksan T, Zekeriyaoglu A, Ak I (2007) The growth of Spirulina platensis in different culture systems under greenhouse condition. Turk J Biol 31:47–52Google Scholar
  34. Goldman JC, Peavey DG (1979) Steady-state growth and chemical composition of the marine Chlorophyte Dunaliella tertiolecta in nitrogen limited continuous cultures. Appl Environ Microb 38:894–901Google Scholar
  35. Greque de Morais M, Vieira Costa JA (2007) Biofixation of carbon dioxide by Spirulina sp. and Scenedesmus obliquus cultivated in a three-stage serial tubular photobioreactor. J Biotechnol 129:439–445CrossRefGoogle Scholar
  36. Grobbelaar JU (2000) Physiological and technological considerations for optimising mass algal cultures. J Appl Phycol 12:201–206CrossRefGoogle Scholar
  37. Harrington KJ (1986) Chemical and physical properties of vegetable oil esters and their effect on diesel fuel performance. Biomass 9:1–17CrossRefGoogle Scholar
  38. Haury JF, Spiller H (1981) Fructose uptake and influence on growth of and nitrogen fixation by Anabaena variabilis. J Bacteriol 147:227–235PubMedGoogle Scholar
  39. Hu G, Gao K (2003) Optimisation of growth and fatty acid composition of a unicellular marine picoplankton, Nannochloropsis sp., with enriched carbon sources. Biotech Lett 25:421–425CrossRefGoogle Scholar
  40. Illman AM, Scragg AH, Shales SW (2000) Increase in Chlorella strains calorific values when grown in low nitrogen medium. Enzyme Microb Tech 27:631–635CrossRefGoogle Scholar
  41. Ishida Y, Hiragushi N, Kitaguchi H, Mitsutani A, Nagai S, Yoshimura M (2000) A highly CO2-tolerant diatom, Thalassiosira weissflogii H1, enriched from coastal sea, and its fatty acid composition. Fish Sci 66:655–659CrossRefGoogle Scholar
  42. Janssen M, Slenders P, Tramper J, Mur LR, Wijffels RH (2001) Photosynthetic efficiency of Dunaliella tertiolecta under short light/dark cycles. Enzyme Microb Tech 29:298–305CrossRefGoogle Scholar
  43. Johansen J, Lemke P, Nagle N, Chelf P, Roessler P, Galloway R, Toon S (1987) Addendum to microalga culture collection 1986–1987. Report prepared by the SERI Microalgal Technology Research Group, Golden, Colorado. Report number SERI/SP-232-3079a, dated December 1987Google Scholar
  44. Lee Y-K (2001) Microalgal mass culture systems and methods: their limitations and potential. J Appl Phycol 13:307–315CrossRefGoogle Scholar
  45. Lee ETY, Bazin MJ (1991) Environmental factors influencing photosynthetic efficiency of the micro red alga Porphyridium cruentum (Agardh) Nägeli in light-limited cultures. New Phytol 118:513–519CrossRefGoogle Scholar
  46. Li Y, Horsman M, Wang B, Wu N, Lan CQ (2008) Effects of nitrogen sources on cell growth and lipid accumulation of green alga Neochloris oleoabundans. Appl Microbiol Biotechnol 81:629–636 PubMedCrossRefGoogle Scholar
  47. Liu Z-Y, Wang G-C, Zhou B-C (2008) Effect of iron on growth and lipid accumulation in Chlorella vulgaris. Bioresour Technol 99:4717–4722PubMedCrossRefGoogle Scholar
  48. Lu C, Rao K, Hall D, Vonshak A (2001) Production of eicosapentanoic acid (EPA) in Monodus subterraneus grown in a helical tubular photobioreactor as affected by cell density and light intensity. J Appl Phycol 13:517–522CrossRefGoogle Scholar
  49. Ma F, Hanna A (1999) Biodiesel production: a review. Bioresour Technol 70:1–15CrossRefGoogle Scholar
  50. Maddux WS, Jones RF (1964) Some interactions of temperature, light intensity and nutrient concentration during the continuous culture of Nitzschia closterium and Tetraselmis sp. Limnol Oceanogr 9:79–86CrossRefGoogle Scholar
  51. Mansour MP, Frampton DMF, Nichols PD, Volkman JK (2005) Lipid and fatty acid yield of nine stationary-phase microalgae: applications and unusual C24–C28 polyunsaturated fatty acids. J Appl Phycol 17:287–300CrossRefGoogle Scholar
  52. Matsukawa R, Hotta M, Masuda Y, Chihara M, Karube I (2000) Antioxidants from carbon dioxide fixing Chlorella sorokiniana. J Appl Phycol 12:263–267CrossRefGoogle Scholar
  53. McGinnis KM, Dempster TA, Sommerfield MR (1997) Characterization of the growth and lipid content of the diatom Chaetoceros muelleri. J Appl Phycol 9:19–24CrossRefGoogle Scholar
  54. McKnight D (1981) Chemical and biological processes controlling the response of a freshwater ecosystem to copper stress: a field study of the CuSO4 treatment of Mill Pond reservoir, Burlington, Massachusetts. Limnol Oceanogr 26:518–531Google Scholar
  55. Moheimani NR (2005) The culture of Coccolithophorid algae for carbon dioxide bioremediation. PhD Thesis, Murdoch University, Perth, AustraliaGoogle Scholar
  56. Moheimani NR, Borowitzka MA (2006) The long-term culture of the coccolithophore Pleurochrysis carterae (Hapytophyta) in outdoor raceway ponds. J Appl Phycol 18:703–712CrossRefGoogle Scholar
  57. Molina Grima E, Robles Medina A, Gimenez Gimenez A, Sanchez Perez JA, Garcia Camacho F, Garcia Sanchez JL (1994) Comparison between extraction of lipids and fatty acids from microalgal biomass. J Am Oil Chem Soc 71:955–959CrossRefGoogle Scholar
  58. Moore JW (1975) Seasonal changes in the proximate and fatty acid composition of some naturally grown freshwater Chlorophytes. J Phycol 11:205–211Google Scholar
  59. Mourente G, Lubiain LM, Odriozola JM (1990) Total fatty acid composition as a taxonomic index of some marine microalgae used as food in marine aquaculture. Hydrobiologia 203:148–154CrossRefGoogle Scholar
  60. Nagle N, Lemke P (1990) Production of methyl ester fuel from microalgae. Appl Biochem Biotech 24–25:355–361CrossRefGoogle Scholar
  61. Ostgaard K, Jensen A (1982) Diurnal and circadian rhythms in the turbidity of growing Skeletonema costatum cultures. Mar Biol 66:261–268CrossRefGoogle Scholar
  62. Parrish CC, Wangersky PJ (1987) Particulate and dissolved lipid classes in cultures of Phaeodactylum tricornutum grown in cage culture turbidostats with a range of nitrogen supply rates. Mar Ecol-Prog Ser 35:119–128CrossRefGoogle Scholar
  63. Patil V, Kallqvist T, Olsen E, Vogt G, Gislerod HR (2007) Fatty acid composition of 12 microalgae for possible use in aquaculture feed. Aquacult Int 15:1–9CrossRefGoogle Scholar
  64. Piorreck M, Baasch K-L, Pohl P (1984) Biomass production, total protein, chlorophylls, lipids and fatty acids of freshwater green and blue-green algae under different nitrogen regimes. Phytochemistry 23:207–216CrossRefGoogle Scholar
  65. Price LL, Yin K, Harrison PJ (1998) Influence of continuous light and L:D cycles on the growth and chemical composition of Prymnesiophyceae including Coccolithophores. J Exp Mar Biol Ecol 223:223–234CrossRefGoogle Scholar
  66. Pulz O (2001) Photobioreactors: production systems for phototrophic microorganisms. Appl Microbiol Biotechnol 57:287–293PubMedCrossRefGoogle Scholar
  67. Pulz O, Gross W (2004) Valuable products from biotechnology of microalgae. Appl Microbiol Biotechnol 65:635–648PubMedCrossRefGoogle Scholar
  68. Qiang H, Guterman H, Richmond A (1996) Physiological characteristics of Spirulina platensis (Cyanobacteria) cultured at ultrahigh cell densities. J Phycol 32:1066–1073CrossRefGoogle Scholar
  69. Reitan KI, Rainuzzo JR, Olsen Y (1994) Effect of nutrient limitation on fatty acid and lipid content of marine microalgae. J Phycol 30:972–979CrossRefGoogle Scholar
  70. Renaud SM, Parry DL, Luoing-Van Thinh (1994) Microalgae for use in tropical aquaculture I: Gross chemical and fatty acid composition of twelve species of microalgae from the Northern Territory, Australia. J Appl Phycol 6:337–345CrossRefGoogle Scholar
  71. Richardson B, Orcutt DM, Shwertner A, Martinez AL, Wickline HE (1969) Effects of nitrogen limitation on the growth and composition of unicellular algae in continuous culture. Appl Microbiol 18:245–250PubMedGoogle Scholar
  72. Rodolfi L, Zittelli GC, Bassi N, Padovani G, Biondi N, Bonini G, Tredici MR (2008) Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor. Biotechnol Bioeng 102(1):100–112CrossRefGoogle Scholar
  73. Roessler PG (1990) Environmental control of glycerolipid metabolism in microalgae: commercial implications and future research directions. J Phycol 26:393–399CrossRefGoogle Scholar
  74. Sheehan J, Dunahay T, Benemann J, Roessler P (1998) A look back at the U.S. Department of Energy’s Aquatic Species Program: Biodiesel from Algae. Close-Out report. National Renewable Energy Lab, Department of Energy, Golden, Colorado, U.S.A. Report number NREL/TP-580-24190, dated July 1998Google Scholar
  75. Shehata TE, Kempner ES (1977) Growth and cell volume of Euglena gracilis in different media. Appl Environ Microbiol 33:374–877Google Scholar
  76. Shifrin NS, Chisholm SW (1981) Phytoplankton lipids: interspecific differences and effects of nitrate, silicate and light–dark cycles. J Phycol 17:374–384CrossRefGoogle Scholar
  77. Siron R, Giusti G, Berland B (1989) Changes in the fatty acid composition of Phaeodactylum tricornutum and Dunaliella tertiolecta during growth and under phosphorous deficiency. Mar Ecol-Prog Ser 55:95–100CrossRefGoogle Scholar
  78. Sorokin C, Krauss RW (1961) Effects of temperature and illuminance on Chlorella growth uncoupled from cell division. Plant Physiol 37:37–42CrossRefGoogle Scholar
  79. Spoehr HA, Milner HW (1949) The chemical composition of Chlorella, effect of environmental conditions. Plant Physiol 24:120–149PubMedCrossRefGoogle Scholar
  80. Suen Y, Hubbard JS, Holzer G, Tornabene TG (1987) Total lipid production of the green alga Nannochloropsis sp. QII under different nitrogen regimes. J Phycol 23:289–296CrossRefGoogle Scholar
  81. Taguchi S, Hirata JA, Laws EA (1987) Silicate deficiency and lipid synthesis of marine diatoms. J Phycol 23:260–267CrossRefGoogle Scholar
  82. Tomaselli L, Boldrini G, Margheri MC (1997) Physiological behaviour of Arthrospira (Spirulina) maxima during acclimation to changes in irradiance. J Appl Phycol 9:37–43CrossRefGoogle Scholar
  83. Tsukahara K, Sawayama S (2005) Liquid fuel production using microalgae. J Jpn Pet Inst 48(5):251–259CrossRefGoogle Scholar
  84. Ugwu CH, Aoyagi H, Uchiyama H (2007) Influence of irradiance, dissolved oxygen concentration and temperature on the growth of Chlorella sorokiniana. Photosynthetica 45:309–311CrossRefGoogle Scholar
  85. Vieira Costa JA, Colla LM, Filho PD, Kabke K, Weber A (2002) Modelling of Spirulina platensis growth in fresh water using response surface methodology. World J Microb Biotechnol 18:603–607CrossRefGoogle Scholar
  86. Xu H, Miao X, Wu Q (2006) Biodiesel production from heterotrophic microalgal oil. Bioresour Technol 97:841–846CrossRefGoogle Scholar
  87. Zeiler KG, Heacox DA, Toon ST, Brown LM (1995) Proceedings of the 1995 meeting of the Phycological Society of America, Breckenridge, 6–10 August 1995. J Phycol (suppl 31):9Google Scholar

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© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Centre for Bioprocess Engineering ResearchUniversity of Cape TownCape TownSouth Africa

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