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Journal of Applied Phycology

, Volume 21, Issue 4, pp 413–422 | Cite as

Cultivation of the brown alga Sargassum horneri: sexual reproduction and seedling production in tank culture under reduced solar irradiance in ambient temperature

  • Shao Jun PangEmail author
  • Feng Liu
  • Ti Feng Shan
  • Su Qin Gao
  • Zhi Huai Zhang
Article

Abstract

As a large conspicuous intertidal brown alga, individuals of Sargassum horneri can reach a length of more than 7 m with a fresh weight of 3 kg along the coasts of the Eastern China Sea. The biomass of this alga as a vital component in coastal water ecology has been well documented. In recent years, a steady disappearance of the algal biomass along the once densely populated coastal areas of the Eastern China Sea has drawn attention in China. Efforts have been made to reconstruct the subtidal algal flora or even to grow the alga by use of long-lines. As part of the efforts to establish an efficient technique for producing seedlings of S. horneri, in this investigation a series of culture experiments were carried out in indoor raceway and rectangular tanks under reduced solar irradiance at ambient temperature in 2007–2008. The investigation demonstrated that: (1) sexual reproduction of S. horneri could be accelerated in elevated temperature and light climates, at least 3 months earlier than in the wild; (2) eggs of S. horneri had the potential to be fertilized up to 48 h, much longer than that of known related species; (3) suspension and fixed culture methods were both effective in growing the seedlings to the long-line cultivation stage; and (4) the life cycle of S. horneri in culture could be shortened to 4.5 months, thus establishing this alga as an appropriate model for investigating sexual reproduction in dieocious species of this genus.

Keywords

Sargassum horneri Fertilization Brown alga Seaweed cultivation Seedling production 

Notes

Acknowledgements

The authors wish to thank C.H. Song for her technical assistance in maintaining the algal culture in the lab. This work is financially supported by: (1) the 863 Hi-Tech Research and Development Program of China (2006AA10A412, 2006AA10A416); (2) a project from Natural Science Foundation of China (30671596); (3) a project from the Chinese Academy of Sciences (KSCX2-YW-N-47–07); and (4) a project from the Ministry of Science and technology of China (2006GB24910469).

References

  1. Arenas F, Fernadéz C, Rico JM, Fernandéz E, Haya D (1995) Growth and reproductive strategies of Sargassum muticum (Yendo) Fensholt and Cystoseira nodicaulis (Whit.) Roberts. Sci Mar 59:1–8Google Scholar
  2. Berndt ML, Callow JA, Brawley SH (2002) Gamete concentrations and timing and success of fertilization in a rocky shore seaweed. Mar Ecol Prog Ser 226:273–285. doi: 10.3354/meps226273 CrossRefGoogle Scholar
  3. Choi CG, Kim HG, Sohn CH (2003) Transplantation of young fronds of Sargassum horneri for construction of seaweed beds. J Korean Fish Soc 36:469–473Google Scholar
  4. Choi HG, Lee KH, Yoo HI, Kang PJ, Kim YS, Nam KW (2008) Physiological differences in the growth of Sargassum horneri between the germling and adult stages. J Appl Phycol. doi: 10.1007/s10811-007-9281-5
  5. Critchley AT, Ohno M (1998) Seaweed resources of the world. Japan International Cooperation Agency, YokosukaGoogle Scholar
  6. Gao KS, Hua WQ (1997) In situ growth rates of Sargassum horneri (Fucales, Phaeophyta). Phycol Res 45:55–57. doi: 10.1111/j.1440-1835.1997.tb00062.x CrossRefGoogle Scholar
  7. Hwang EK, Park CS, Baek JM (2006) Artificial seed production and cultivation of the edible brown alga, Sargassum fulvellum (Turner) C. Agardh: developing a new species for seaweed cultivation in Korea. J Appl Phycol 18:251–257. doi: 10.1007/s10811-006-9021-2 CrossRefGoogle Scholar
  8. Komatsu T, Matsunaga D, Mikami A, Sagawa T, Boisnier E, Tatsukawa K, Aoki M, Ajisaka T, Uwai S, Tanaka K, Ishida K, Tanoue H, Sugimoto T (2008) Abundance of drifting seaweeds in eastern East China Sea. J Appl Phycol. doi: 10.1007/s10811-007-9302-4
  9. Lüning K, Kadel P, Pang SJ (2008) Control of reproduction rhythmicity by environmental and endogenous signals in Ulva pseudocurvata (Chlorophyta). J Phycol 44:866–873. doi: 10.1111/j.1529-8817.2008.00535.x CrossRefGoogle Scholar
  10. Nanba N (1995) Egg release and germling development in Sargassum horneri (Fucales, Phaeophyceae). Phycol Res 43:121–125. doi: 10.1111/j.1440-1835.1995.tb00014.x CrossRefGoogle Scholar
  11. Pang SJ, Lüning K (2004) Breaking seasonal limitation: year-round induction of sporogenesis of Laminaria saccharina by blocking the transport of sporulation inhibiting factors. Aquaculture 240:531–541. doi: 10.1016/j.aquaculture.2004.06.034 CrossRefGoogle Scholar
  12. Pang SJ, Lüning K (2006) Tank cultivation of the red alga Palmaria palmata: year-round induction of tetrasporangia, tetraspore release in darkness and mass cultivation of vegetative thalli. Aquaculture 252:20–30. doi: 10.1016/j.aquaculture.2005.11.046 CrossRefGoogle Scholar
  13. Pang SJ, Chen LT, Zhuang DG, Fei XG, Sun JZ (2005) Cultivation of the brown alga Hizikia fusiformis (Harvey) Okamura: enhanced seedling production in tumbled culture. Aquaculture 245:321–329. doi: 10.1016/j.aquaculture.2004.12.011 CrossRefGoogle Scholar
  14. Pang SJ, Gao SQ, Sun JZ (2006) Cultivation of the brown alga Hizikia fusiformis (Harvey) Okamura: controlled fertilization and early development of seedlings in raceway tanks in ambient light and temperature. J Appl Phycol 18:723–731. doi: 10.1007/s10811-006-9078-y CrossRefGoogle Scholar
  15. Pang SJ, Zhang ZH, Shan TF, Gao SQ, Sun JZ (2007) Cultivation of the brown alga Hizikia fusiformis (Harvey) Okamura: stress resistance of artificially raised young seedlings revealed by chlorophyll fluorescence measurement. J Appl Phycol 19:557–565. doi: 10.1007/s10811-007-9170-y CrossRefGoogle Scholar
  16. Stæhr PA, Pedersen MF, Thomsen MS, Wernberg T, Krause-Jensen D (2000) Invasion of Sargassum muticum in Limfjorden (Denmark) and its possible impact on the indigenous macroalgal community. Mar Ecol Prog Ser 207:79–88. doi: 10.3354/meps207079 CrossRefGoogle Scholar
  17. Sun JZ, Chen WD, Zhuang DG, Zheng HY, Li L, Pang SJ (2008) In situ ecological studies of the subtidal brown alga Sargassum horneri at Nanji Island of China. S China Fish Sci 4(3):59–64. in Chinese with English abstractGoogle Scholar
  18. Uchida T (1993) The life cycle of Sargassum horneri {Phaeophyta) in laboratory culture. J Phycol 29:231–235. doi: 10.1111/j.0022-3646.1993.00231.x CrossRefGoogle Scholar
  19. Uchida T, Arima S (1993) Crossing experiments between autumn- and spring-fruiting types of Sargassum horneri (Phaeophyta). Bull Jpn Soc Sci Fish 59:1685–1688Google Scholar
  20. Yamauchi K (1984) The formation of Sargassum beds on artificial substrata by transplanting seedlings of S. horneri (Turner) C. Agardh and S. muticum (Yendo) Fensholt. Bull Jpn Soc Sci Fish 50:1115–1123Google Scholar
  21. Yoshida G, Arima S, Terawaki T (1998) Growth and maturation of the “autumn-fruiting type” of Sargassum horneri (Fucales, Phaeophyta) and comparisons with the “spring-fruiting type”. Phycol Res 46:183–189. doi: 10.1111/j.1440-1835.1998.tb00112.x CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Shao Jun Pang
    • 1
    Email author
  • Feng Liu
    • 1
    • 2
  • Ti Feng Shan
    • 1
    • 2
  • Su Qin Gao
    • 1
  • Zhi Huai Zhang
    • 1
  1. 1.Marine Biological Culture Collection Centre, Institute of OceanologyChinese Academy of SciencesQingdaoChina
  2. 2.Graduate University of Chinese Academy of SciencesBeijingChina

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