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Journal of Applied Phycology

, Volume 20, Issue 3, pp 245–251 | Cite as

Effects of light intensity and nitrogen starvation on growth, total fatty acids and arachidonic acid in the green microalga Parietochloris incisa

  • A. E. Solovchenko
  • I. Khozin-Goldberg
  • S. Didi-Cohen
  • Z. Cohen
  • M. N. Merzlyak
Article

Abstract

The effects of light and nitrogen deficiency on biomass, fatty acid content and composition were studied in Parietochloris incisa, the unicellular freshwater chlorophyte accumulating very high amounts of arachidonic-acid-rich triacylglycerols. P. incisa cultures grown on complete nutrient medium and under high light (400 μmol photons m− 2 s−1) showed the highest rate of growth in comparison to medium (200 μmol photons m−2 s−1) and low (35 μmol photons m−2 s−1) light intensity. Cultures grown under high light (on complete BG-11 medium) attained higher volumetric contents of total fatty acids and arachidonic acid due to greater increase in biomass. Nitrogen starvation brought about a strong increase in the arachidonic acid proportion of total fatty acids. Thus, adjustments to cultivation conditions could serve as an efficient tool for manipulation of yield and relative content of arachidonic acid in P. incisa. The significance of the changes in lipid metabolism for adaptation of P. incisa to high-light stress and nitrogen deficiency is also discussed.

Keywords

Alga cultivation Lipids Microalgal biotechnology Photoadaptation 

Abbreviations

AA

Arachidonic acid

DW

Dry weight

FA

Fatty acids

PFD

Photon Flux Density

TAG

Triacylglycerols

TFA

Total fatty acids

PUFA

Polyunsaturated fatty acids

Notes

Acknowledgements

This work was supported in part by fellowships from the BIDR to M.N.M. and A.E.S. M.N.M. and A.E.S. also acknowledge the financial support of the Russian Fund for Basic Research (Grant # 06-04-48883) and Russia President’s Grant Council (Ministry of Science of the Russian Federation).

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • A. E. Solovchenko
    • 1
  • I. Khozin-Goldberg
    • 2
  • S. Didi-Cohen
    • 2
  • Z. Cohen
    • 2
  • M. N. Merzlyak
    • 1
  1. 1.Department of Physiology of Microorganisms, Faculty of BiologyMoscow State UniversityMoscowRussia
  2. 2.Microalgal Biotechnology Laboratory, The Albert Katz Department of Dryland Biotechnologies, The Jacob Blaustein Institutes for Desert ResearchBen-Gurion University of the NegevSede-BokerIsrael

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