Journal of Applied Phycology

, Volume 20, Issue 2, pp 161–168 | Cite as

Mitochondrial cox1 and plastid rbcL genes of Gracilaria vermiculophylla (Gracilariaceae, Rhodophyta)

  • Eun Chan Yang
  • Myung Sook Kim
  • Paul John L. Geraldino
  • Dinabandhu Sahoo
  • Jong-Ahm Shin
  • Sung Min Boo


The mitochondrial cytochrome c oxidase subunit I gene sequence was recently developed for DNA barcoding of red algal species. We determined the 1245 base pairs of the gene from 27 taxa of an agar-producing species, Gracilaria vermiculophylla, and putative relatives and compared the results with rbcL data from the same species. A total of 392 positions (31.5%) were variable, 282 positions (22.6%) were parsimoniously informative, and average sequence divergence was 13% in an ingroup. Within G. vermiculophylla, pairwise divergence of the gene was variable up to 11 bp (0.9%). Seven recognized haplotypes of cox1 tended to be geographically related. In the aligned 1386 bp of rbcL, three haplotypes were recognized. These results suggest that cox1 is a valuable molecular marker within species and will be very useful in haplotype analyses.


DNA barcoding Gracilariales Haplotype Molecular marker Phylogeny 



This research was supported by the Marine and Extreme Genome Research Center Program (program leader: Dr. S. J. Kim), Ministry of Maritime Affairs & Fisheries, Korea, to S. M. Boo.


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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Eun Chan Yang
    • 1
  • Myung Sook Kim
    • 2
  • Paul John L. Geraldino
    • 1
  • Dinabandhu Sahoo
    • 3
  • Jong-Ahm Shin
    • 4
  • Sung Min Boo
    • 1
  1. 1.Department of BiologyChungnam National UniversityDaejeonSouth Korea
  2. 2.Research Institute of Basic SciencesPusan National UniversityPusanSouth Korea
  3. 3.Marine Biotechnology Laboratory, Department of BotanyUniversity of DelhiDelhiIndia
  4. 4.Fisheries and Ocean ScienceChonnam National UniversityYeosuSouth Korea

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