Mitochondrial cox1 and plastid rbcL genes of Gracilaria vermiculophylla (Gracilariaceae, Rhodophyta)
The mitochondrial cytochrome c oxidase subunit I gene sequence was recently developed for DNA barcoding of red algal species. We determined the 1245 base pairs of the gene from 27 taxa of an agar-producing species, Gracilaria vermiculophylla, and putative relatives and compared the results with rbcL data from the same species. A total of 392 positions (31.5%) were variable, 282 positions (22.6%) were parsimoniously informative, and average sequence divergence was 13% in an ingroup. Within G. vermiculophylla, pairwise divergence of the gene was variable up to 11 bp (0.9%). Seven recognized haplotypes of cox1 tended to be geographically related. In the aligned 1386 bp of rbcL, three haplotypes were recognized. These results suggest that cox1 is a valuable molecular marker within species and will be very useful in haplotype analyses.
KeywordsDNA barcoding Gracilariales Haplotype Molecular marker Phylogeny
This research was supported by the Marine and Extreme Genome Research Center Program (program leader: Dr. S. J. Kim), Ministry of Maritime Affairs & Fisheries, Korea, to S. M. Boo.
- Freshwater DW, Rueness J (1994) Phylogenetic relationships of some European Gelidium (Gelidiales, Rhodophyta) species based on rbcL nucleotide sequence analysis. Phycologia 33:187–194Google Scholar
- Lee IK, Kim MS, Boo SM (1995) A taxonomic appraisal of Korean Gracilaria verrucosa (Rhodophyta, Gracilariales). In: Abbott IA (ed) Taxonomy of economic seaweeds with reference to some Pacific species. California Sea Grant, California, pp 213–222Google Scholar
- Lim P-E, Wong C-L, Phang S-M (2006) Molecular taxonomy of seaweeds with emphasis on Rhodophyta and Phaeophyta. In: Phang S-M, Critchley AT, Ang PO Jr (eds) Advances in seaweed cultivation and utilization in Asia. University of Malaya, Kuala Lumpur, pp 105–142Google Scholar
- Ohmi H (1956) Contributions to the knowledge of Gracilariaceae from Japan. II. On a new species of the genus Gracilariopsis, with some considerations on its ecology. Bull Fac Fish Hokkaido Univ 6:271–279Google Scholar
- Swofford DL (2002) PAUP*: phylogenetic analysis using parsimony (*and other methods). Version 4.0b10. Sinauer Associates, Sunderland, MassachusettsGoogle Scholar
- Terada R, Ohno M (2000) Notes on Gracilaria (Gracilariales, Rhodophyta) from Tosa Bay and adjacent waters I: Gracilaria chorda, Gracilaria gigas and Gracilaria incurvata. Bull Mar Sci Fish Kochi Univ 20:81–88Google Scholar
- Terada R, Yamamoto H (2002) Review of Gracilaria vermiculophylla and other species in Japan and Asia. In: Abbott IA, McDermid K (eds) Taxonomy of economic seaweeds, with reference to some Pacific species, Vol 8. California Sea Grant College System, USA, pp 215–224Google Scholar
- Yamamoto H (1973) Gracilariopsis chorda (Holm.) Ohmi from Hakodate Bay. Bull Jap Soc Phycol 21:8–91Google Scholar