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Journal of Applied Phycology

, Volume 20, Issue 1, pp 97–102 | Cite as

Tank cultivation of Ulva prolifera in deep seawater using a new “germling cluster” method

  • Masanori HiraokaEmail author
  • Naohiro Oka
Article

Abstract

A new “germling cluster” method is proposed for tank cultivation of seaweed in a free-floating form. This method was applied to the tank cultivation of Ulva prolifera using deep seawater (DSW) pumped up from over 300 m depth off the cape of Muroto in southwest Japan. Numerous zoids of U. prolifera were induced by cutting thalli into 1–2 mm long pieces. Three days after fragment production, the zoids were released. The zoid suspension was concentrated to a density of more than 104 zoids per mL medium, and placed in a Petri dish for culture. The dense, germinating zoids began to adhere to each other and form aggregations. The germling aggregations were then removed from the bottom of the dish and torn into a large number of small “germling clusters” using an electric mixer. Each cluster contained 10–100 germlings. Once the germling clusters had attained more than 5 mm diameter in culture, they were transplanted as free-floating forms to a 500 L outdoor tank with continuous aeration, to which DSW was supplied at an exchange rate of 3 volumes per day. As a result, the average daily growth rate (DGR) in the tank throughout the year was 37%, though the DGR fluctuated with seasonal temperature changes.

Keywords

Chlorophyta Deep seawater Free-floating form “Germling cluster” Tank cultivation Ulva prolifera 

Notes

Acknowledgments

The authors wish to thank the staff of Kochi Prefectural Deep Seawater Laboratory for their cooperation during this study; Dr. Masao Migita (Faculty of Education, Shiga University, Japan) for statistic analysis; and Stephan Gale (Kochi Prefectural Makino Botanical Garden, Japan) and Felix Bast (Usa Marine Biological Institute, Kochi University, Japan) for correcting the English manuscript. This study was partly supported by a NEDO fellowship.

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  1. 1.Usa Marine Biological InstituteKochi UniversityTosaJapan
  2. 2.Sea Lab Ltd (Umi-no-kenkyusha)MurotoJapan

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