Journal of Applied Phycology

, Volume 20, Issue 1, pp 9–12

RNA Isolation method for polysaccharide rich algae: agar producing Gracilaria tenuistipitata (Rhodophyta)

  • Vanessa Dos Reis Falcão
  • Angela Pedroso Tonon
  • Mariana Cabral Oliveira
  • Pio Colepicolo
Article

DOI: 10.1007/s10811-007-9174-7

Cite this article as:
Dos Reis Falcão, V., Pedroso Tonon, A., Cabral Oliveira, M. et al. J Appl Phycol (2008) 20: 9. doi:10.1007/s10811-007-9174-7

Abstract

RNA isolation is essential to study gene expression at the molecular level. However, RNA isolation is difficult in organisms (plants and algae) that contain large amounts of polysaccharides, which co-precipitate with RNA. Currently, there is no commercial kit available, specifically for the isolation of high-quality RNA from these organisms. Furthermore, because of the large amounts of polysaccharides, the common protocols for RNA isolation usually result in poor yields when applied to algae. Here we describe a simple method for RNA isolation from the marine red macroalga Gracilaria tenuistipitata var. liui Zhang et Xia (Rhodophyta), which can be applied to other plants and algae.

Keywords

Gracilaria tenuistipitata Polysaccharides RNA extraction 

Abbreviations

DEPC

Diethylpyrocarbonate

ETOH

Ethanol

GHCL

guanidinium Hydrochloride extraction buffer

HMW-PEG

High molecular weight polyethylene glycol

KOAc

Potassium acetate

PVP

Polyvinylpyrrolidone

RT-PCR

Reverse transcription coupled with polymerase chain reaction

Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  • Vanessa Dos Reis Falcão
    • 1
    • 3
  • Angela Pedroso Tonon
    • 1
  • Mariana Cabral Oliveira
    • 2
  • Pio Colepicolo
    • 1
  1. 1.Departamento de BioquímicaUniversidade de São Paulo, Instituto de QuímicaSão PauloBrazil
  2. 2.Departamento de BotânicaUniversidade de São Paulo, Instituto de BiociênciasSão PauloBrazil
  3. 3.Departamento de BioquímicaIQUSPSão PauloBrazil

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