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Journal of Applied Phycology

, Volume 19, Issue 3, pp 263–270 | Cite as

Method for the quantification of the blue-green pigment “marennine” synthesized by the marine diatom Haslea ostrearia (Gaillon/Bory) Simonsen using HPLC gel-filtration and photodiode-array detection

  • Jean-Bernard Pouvreau
  • Michèle Morançais
  • Joël Fleurence
  • Pierre PondavenEmail author
Article

Abstract

This paper describes a new approach for quantifying marennine, a blue-green pigment synthesized by the marine diatom Haslea ostrearia, which is known to be responsible for the greening of cultured oysters in French coastal areas. The method uses gel-filtration HPLC interfaced with a photodiode-array detector (PDA). Under the chromatographic conditions applied, the peak of marennine is identified on the dextran pattern at 674 nm by its elution time (ET = 21–22 min) and its UV-Visible absorption spectrum. After calibration with pure marennine sample as external standard, consisting in plotting peak area as a function of marennine concentration, one can back-calculate concentrations of unknown samples. Results for quantitative determination of marennine using this procedure are validated and discussed in relation to those obtained by the spectrophotometric methods, the most often employed until now.

Key words

microalga oyster Haslea ostrearia photodiode-array detection marennine pigment 

Abbreviations

EMn

Pure extracellular marennine

IMn

Pure intracellular marennine

PDA

Photodiode-array

CE

Crude extract

LOD

Limit of detection

LOQ

Limit of quantification

ET

Elution time

Notes

Acknowledgments

We gratefully thank Dr. Gary German who kindly revised the English of this manuscript. This research was supported by a Valbiomar European Grant.

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Jean-Bernard Pouvreau
    • 1
  • Michèle Morançais
    • 1
  • Joël Fleurence
    • 1
  • Pierre Pondaven
    • 1
    Email author
  1. 1.Université de Nantes, Nantes Atlantique UniversitésNantes cedex 3France

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