Journal of Applied Phycology

, Volume 18, Issue 3–5, pp 445–450 | Cite as

A Simple 96-Well Microplate Method for Estimation of Total Polyphenol Content in Seaweeds

  • Qing Zhang
  • Junzeng ZhangEmail author
  • Jingkai Shen
  • Angelica Silva
  • Dorothy A. Dennis
  • Colin J. Barrow


Seaweed polyphenols are potent antioxidants and have also been shown to have α-glucosidase inhibiting activity. In our continuous efforts to develop new marine-based nutraceuticals and functional food ingredients, we have investigated many algal species collected on the Atlantic coast of Canada. A simple method for estimating the total polyphenol content in seaweeds and their extracts was developed based on the classic Folin-Ciocalteau colorimetric reaction. By using the 96-well microplate and a microplate reader, this new method saves experimental time, significantly reduces the amount of sample required, handles large number of samples in one experiment, and also improves the repeatability of the results.

A number of algal samples collected on the seashore of Nova Scotia, Canada, were analyzed for their levels of polyphenol content using this microplate-based method. The antioxidant activity of these samples was also assessed by using DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical scavenging assay. The results showed that there is a strong correlation between the total polyphenol content and the potency of antioxidant effect.

Key words

seaweed marine algae total polyphenol estimation 96-well microplate 


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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Qing Zhang
    • 1
  • Junzeng Zhang
    • 1
    Email author
  • Jingkai Shen
    • 1
  • Angelica Silva
    • 1
  • Dorothy A. Dennis
    • 1
  • Colin J. Barrow
    • 1
  1. 1.Ocean Nutrition Canada Ltd.DartmouthCanada

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