Journal of Applied Phycology

, Volume 18, Issue 3, pp 369–373

A comparative study of specificity of fucoidanases from marine microorganisms and invertebrates

  • M. I. Kusaykin
  • A. O. Chizhov
  • A. A. Grachev
  • S. A. Alekseeva
  • I. Yu Bakunina
  • O. I. Nedashkovskaya
  • V. V. Sova
  • T. N. Zvyagintseva
Article

DOI: 10.1007/s10811-006-9042-x

Cite this article as:
Kusaykin, M.I., Chizhov, A.O., Grachev, A.A. et al. J Appl Phycol (2006) 18: 369. doi:10.1007/s10811-006-9042-x

Abstract

Specificities of actions of fucoidanases from the marine microorganism Pseudoalteromonas citrea KMM 3296 and the marine mollusk Littorina kurila were studied. The enzymes possess similar specificities and catalyze the cleavage of accessible α-(1→3)-fucoside bonds in fucoidans with highly sulfated α-(1→4; 1→3)-L-fucooligosaccharides. A high degree of sulfation of the fucose residues in fucoidans makes α-(1→3)-L-fucoside bonds inaccessible for the action of the studied enzymes. The maximum degree of cleavage of fucoidan was achieved by the fucoidanase from the marine bacterium Pseudoalteromonas citrea KMM 3296.

Key words

fucoidan fucoidanase marine invertebrates marine bacteria seaweed 

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • M. I. Kusaykin
    • 1
  • A. O. Chizhov
    • 2
  • A. A. Grachev
    • 2
  • S. A. Alekseeva
    • 1
  • I. Yu Bakunina
    • 1
  • O. I. Nedashkovskaya
    • 1
  • V. V. Sova
    • 1
  • T. N. Zvyagintseva
    • 1
  1. 1.Pacific Institute of Bioorganic Chemistry, Far-East Branch of RASVladivostokRussia
  2. 2.N.D. Zelinsky Institute of Organic Chemistry, RASMoscowRussia

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