Journal of Applied Phycology

, Volume 18, Issue 1, pp 9–14 | Cite as

Sulfated polysaccharides from marine green algae Ulva conglobata and their anticoagulant activity

  • Wenjun Mao
  • Xiaoxue Zang
  • Yi Li
  • Huijuan Zhang


Sulfated polysaccharides from the green algae Ulva conglobata were isolated and prepared by extraction in hot water, precipitation with ethanol and purification by ion-exchange and size-exclusion column chromatography. The characterizations of the sulfated polysaccharides were defined, and containing 23.04–35.20% sulfate ester groups, 10.82–14.91% uronic acid and 3.82–4.51% protein. Gas chromatography analysis shows that the sulfated polysaccharides from Ulva conglobata are mainly consisted of rhamnose with variable contents of glucose and fucose, trace amounts of xylose, glactose and mannose. The anticoagulant properties of the sulfated polysaccharides were compared with those of heparin by studying the activated partial thromboplastin time using normal human plasma. The sulfated polysaccharide from Ulva conglobata collected in Qingdao, China is the most potent among the sulfated polysaccharides tested. The mechanism of anticoagulant activity mediated by the sulfated polysaccharides is due to the direct inhibition of thrombin and the potentiation of heparin cofactor II.

Key words

sulfated polysaccharide Ulva conglobata anticoagulant heparin cofactor II 


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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Wenjun Mao
    • 1
  • Xiaoxue Zang
    • 1
  • Yi Li
    • 2
  • Huijuan Zhang
    • 1
  1. 1.Institute of Marine Drugs and FoodsOcean University of ChinaQingdaoP.R China
  2. 2.401 Hospital of PLAQingdaoP.R. China

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