Journal of Applied Phycology

, Volume 17, Issue 4, pp 349–354 | Cite as

Chemical characterization and radioprotective effect of polysaccharide from Monostroma angicava (Chlorophyta)

  • Wenjun Mao
  • Yi Li
  • Ligen Wu
  • Haiqing Wang
  • Yi Zhang
  • Xiaoxue Zang
  • Huijuan Zhang
Article

Abstract

Polysaccharide from the green alga Monostroma angicava was extracted with boiling water and was purified by ion-exchange and size-exclusion column chromatography. The radioprotective effect of the polysaccharide was investigated in mice. The results show that polysaccharide from M. angicava has a different chemical composition to other Chlorophyta having a high rhamnose – containing sulfated polysaccharide. The sulfate ester content was estimated to be 21.8%. When the polysaccharide was applied to BALB/c mice following whole-body X-ray irradiation the counts of leukocytes, thrombocytes and erythrocytes recovered more rapidly in the polysaccharide treated mice after irradiation. In the irradiated mice, the polysaccharide significantly increased the spleen index, natural killer cytostatic activity and the transformation response of splenic lymphocytes. The present observations suggest that polysaccharide from M. angicava led to leukocytogensis and hematopoetic activation in mice after irradiation and that the biological response might be caused by immune activation.

Keywords

chemical characterization immune activation Monostroma angicava polysaccharide radioprotective effect 

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Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Wenjun Mao
    • 1
  • Yi Li
    • 2
  • Ligen Wu
    • 1
  • Haiqing Wang
    • 2
  • Yi Zhang
    • 1
  • Xiaoxue Zang
    • 1
  • Huijuan Zhang
    • 1
  1. 1.Institute of Marine Drugs and FoodsOcean University of ChinaQingdaoP.R. China
  2. 2.401 Hospital of PLAQingdaoP.R. China

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