Journal of Applied Phycology

, Volume 16, Issue 6, pp 499–503

Effects of organic carbon sources on cell growth and eicosapentaenoic acid content of Nannochloropsis sp.

Article
  • 969 Downloads

Abstract

A strain of Nannochloropsis isolated originally from the East China Sea and obtained from Institute of Hydrobiology, Chinese Academy of Sciences was shown to utilize glucose or ethanol for mixotrophic and heterotrophic growth. The highest cell density, 550 mg L− 1 dry weight after culture for 8 days, was obtained during mixotrophic culture with 30 mM glucose. The organic carbon sources had no effect on the net photosynthetic rate, but enhanced the respiratory rate. The addition of an organic carbon source led to an increase in the cell lipid content and a decrease in their eicosapentaenoic acid (EPA) content. The EPA yield was 21.9 mg L− 1 using photoautotrophic culture, and 23.4 mg L− 1 and 23.0 mg L− 1, respectively, in mixotrophic cultivation with glucose or ethanol as the carbon source.

Key Words

eicosapentaenoic acid ethanol glucose Nannochloropsis sp. organic carbon sources 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ackman RG, Jangaard PM, Hoyle RJ, Brocherhoff H (1964) Origin of marine fattyacids: I. Analysis of the fatty acids produced by the diatom Skeletonema costatum. J. Fish. Res. Bd Can. 21: 747–756.Google Scholar
  2. Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37: 911–917.PubMedGoogle Scholar
  3. Brown MR, Jeffrey SW, Garland CD (1989) Nutritional aspects of microalgae used in mariculture: A literature review. CSIRO. Marine Laboratories Report 205, CSIRO Australia, I-44.Google Scholar
  4. Carreau JP, Dudacg JP (1978) Adaptation of a macro-scale method to themicro-scale for fatty acid methyl transesterification of biological lipid extracts. J. Chromatogr. 151: 384–390.CrossRefGoogle Scholar
  5. Chen F and Johns MR (1991) Effect of C/N ratio and aeration on the fatty acid composition of heterotrophic Chlorella sorokiniana. J. Appl. Phycol. 3: 203–209.Google Scholar
  6. Collos Y, Mornet F, Sciandra A, Waser N, Larson A, Harrison PJ (1999) An optical method for the rapid measurement of micromolar concentrations of nitrate in marine phytoplankton cultures. J. Appl. Phycol. 11: 179–184.CrossRefGoogle Scholar
  7. Day JG, Tsalavos A (1996) An investigation of heterotrophic culture of the green alga Tetraselmis. J. appl. Phycol. 8: 73–77.Google Scholar
  8. Facundo JM, Sasaki K, Kakizono T, Nishio N, Nagai S (1993) Growth characteristics of Spirulina platensis in mixotrophic and heterotrophic conditions. J. Ferment. Bioeng. 76: 408–410.CrossRefGoogle Scholar
  9. Hall DO, Rao KK (1994) Photosynthesis, 5th ed. Cambridge University Press, Cambridge.Google Scholar
  10. Harrison PJ, Waters RE, Taylor FJR (1980) A broad spectrum artificial seawater medium for coastal and open ocean phytoplankton. J. Phycol. 16: 28–35.CrossRefGoogle Scholar
  11. Henley WJ (1993) Measurement and interpretation of photosynthetic light–response curves in alga in the context of photoinhibition and diel changes. J. Phycol. 29: 729.CrossRefGoogle Scholar
  12. Hu H, Dai L, Dai H, Zhang X (1999) Amino acid and fatty acid composition of a marine species of Xanthophyceae. Chin. J. Appl. Environ. Biol. 5: 487–490.Google Scholar
  13. Lubzens E, Gibson O, Zmora O, and Sukenik A (1995) Potential advantages of frozen algae (Nannochloropsis sp.) for rotifer (Brachionus plicatilis) culture. Aquaculture 133: 295–310.CrossRefGoogle Scholar
  14. Nordoy A (1991) Is there a rational use for n –3 fatty acids (fish oil) in clinical medicine? Drugs 42: 331–342.PubMedGoogle Scholar
  15. Ogawa T, Aiba S (1981) Bioenergetic analysis of mixotrophic growth in Chlorella vulgaris and Scenedesmus acutus. Biotechnol. Bioengng. 23: 1121–1132.CrossRefGoogle Scholar
  16. Ratledge C (1992) Microbial lipids: Commercial realities or academic curiosities. In Kyle DJ, Rateledge C (eds), Industrial Applications of Single Cell Oils. AOCS Press, Champaign, IL, pp. 1–15.Google Scholar
  17. Suen Y, Hubbard JS, Holzer G, Tornabene TG (1987) Total lipid production of the green alga Nannochloropsis sp. QII under different nitrogen regimes. J. Phycol. 23: 289–296.Google Scholar
  18. Tan CK, Johns MR (1996) Screening of diatoms for heterotrophic eicosapentaenoic production. J. appl. Phycol. 8: 59–64.Google Scholar
  19. Wen ZY, Chen F (2000) Production potential of eicosapentaenoic acids by the diatom Nitzschia laevis. Biotechnol. Lett. 22: 727–733.CrossRefGoogle Scholar

Copyright information

© Springer Science + Business Media, Inc. 2004

Authors and Affiliations

  1. 1.State Key Laboratory of Biochemical Engineering, Institute of Process EngineeringChinese Academy of SciencesBeijingChina

Personalised recommendations