Role of Information Provision in Reversed-Phase High-Performance Liquid Chromatography (Exemplified by Impurity Identification in Dihydroquercetin)
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Abstract
It was noted that the common current practice of presenting the results of identifying organic compounds in reversed-phase HPLC with the specification of analytical parameters that do not exhibit interlaboratory reproducibility or without giving any data is inadequate. This approach is oriented only to the use of reference materials, and it actually implies that identification be performed anew in each particular case. The means of solving this general problem based on the use of retention indices and UV-spectroscopic invariants, such as relative absorbance, are exemplified by solving a particular problem of the identification of impurities in dihydroquercetin.
Keywords
Chromatography Analytical Chemistry HPLC Liquid Chromatography Organic Compound
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