[3H]-Flunitrazepam-labeled Benzodiazepine Binding Sites in the Hippocampal Formation in Autism: A Multiple Concentration Autoradiographic Study

  • Jeffrey T. Guptill
  • Anne B. Booker
  • Terrell T. Gibbs
  • Thomas L. Kemper
  • Margaret L. Bauman
  • Gene J. Blatt
Original Paper

Abstract

Increasing evidence indicates that the GABAergic system in cerebellar and limbic structures is affected in autism. We extended our previous study that found reduced [3H]flunitrazepam-labeled benzodiazepine sites in the autistic hippocampus to determine whether this reduction was due to a decrease in binding site number (Bmax) or altered affinity (Kd) to bind to the ligand. Quantitation of hippocampal lamina demonstrated a 20% reduction in Bmax indicating a trend toward a decreased number of benzodiazepine binding sites in the autistic group but normal Kd values. A reduction in the number of hippocampal benzodiazepine binding sites suggests alterations in the modulation of GABAA receptors in the presence of GABA in the autistic brain, possibly resulting in altered inhibitory functioning of hippocampal circuitry.

Keywords

Developmental disorder Autoradiography Hippocampus GABAergic receptors Hippocampal circuitry 

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Copyright information

© Springer Science+Business Media, LLC 2006

Authors and Affiliations

  • Jeffrey T. Guptill
    • 1
  • Anne B. Booker
    • 1
  • Terrell T. Gibbs
    • 2
  • Thomas L. Kemper
    • 1
  • Margaret L. Bauman
    • 1
  • Gene J. Blatt
    • 1
  1. 1.Department of Anatomy and NeurobiologyBoston University School of MedicineBostonUSA
  2. 2.Department of Pharmacology and TherapeuticsBoston University School of MedicineBostonUSA

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