Abstract
Efficient binding of enzymes onto the electrode surface has been prerequisite for the construction of sensitive biosensors and biochips. Here, a simple and robust construction of electrochemical glucose biosensor based on pyrroloquinoline quinone-glucose dehydrogenase was demonstrated. The glucose biosensor was fabricated by binding the enzyme onto the anionic self-assembled monolayers on gold electrode via electrostatic interactions. The resulting glucose biosensor gave rise to twofold higher detection sensitivity than that by covalent conjugation under the same condition. Surface plasmon resonance and atomic force microscopy analyses revealed that electrostatic binding of the enzyme leads to much higher surface density of the enzyme. This approach will find wide applications to the development of robust enzyme-based biosensors and biochips.
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References
Wang J (2008) Chem Rev 108:814
Goswami P, Sarma AK, Vatsyayan P, Minteer SD (2009) Biosens Bioelectron 24:2313
Duine JA, Frank J (1981) Trends Biochem Sci 6:278
Duine JA (1999) J Biosci Bioeng 88:231
Sode K, Ootera T, Shirahane M, Witarto AB, Igarashi S, Yoshida H (2000) Enzyme Microbiol Technol 26:491
Yamazaki T, Kojima K, Sode K (2000) Anal Chem 72:4689
Kano K, Tsujimura S, Ishii A, Abo T (2008) J Electroanal Chem 614:67
Zayats M, Katz E, Baron R, Willner I (2005) J Am Chem Soc 127:12400
Raj CR, Jena BK (2006) Anal Chem 78:6332
Yehezkeli O, Tel-Vered R, Reichlin S, Willner I (2011) ACS Nano 5:2385
Mano N, Flexer V, Durand F, Tsujimura S (2011) Anal Chem 83:5721
Ikebukuro K, Kohiki Y, Sode K (2002) Biosens Bioelectron 17:1075
El-Shora HM, Youssef MM (2008) J Appl Sci Res 4:545
Kricka LJ (1994) Clin Chem 40:347
Decher G (1997) Science 277:1232
Kim HS, Yoon HC (2000) Anal Chem 72:922
Mrksich M, Whitesides GM (1996) Annu Rev Biophys Biomol 25:55
Patel N, Davies MC, Hartshorne M, Heaton RJ, Roberts CJ, Tendler SJB, Williams PM (1997) Langmuir 13:6485
Jiang SY, Li LY, Chen SF (2003) Langmuir 19:2974
Nakano K, Doi K, Tamura K, Katsumi Y, Tazaki M (2003) Chem Commun 13:1544
Gooding JJ, Hibbert DB (1999) Trac Trend Anal Chem 18:525
Guiomar AJ, Guthrie JT, Evans SD (1999) Langmuir 15:1198
Jin W, Bier F, Wollenberger U, Scheller F (1995) Biosens Bioelectron 10:823
Dijkstra BW, Oubrie A, Rozeboom HJ (1999) Proc Natl Acad Sci USA 96:11787
Ryabov AD, Razumiene J, Meskys R, Gureviciene V, Laurinavicius V, Reshetova MD (2000) Electrochem Commun 2:307
Razumiene J, Gureviciene V, Vilkanauskyte A, Marcinkeviciene L, Bachmatova I, Meskys R, Laurinavicius V (2003) Sens Actuator B Chem 95:378
Tanaka S, Igarashi S, Ferri S, Koji S (2005) BMC Biochem 6:1
Durand F, Stines-Chaumeil C, Flexer V, Andre I, Mano N (2010) Biochem Biophys Res Commun 402:750
Kluger R, Alagic A (2004) Bioorg Chem 32:451
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Dijkstra BW, Oubrie A, Rozeboom HJ, Kalk KH, Olsthoorn AJJ, Duine JA (1999) EMBO J 18:5187
Lahiri J, Isaacs L, Tien J, Whitesides GM (1999) Anal Chem 71:777
Hong MY, Yoon HC, Kim HS (2003) Langmuir 19:416
Roth CM, Sader JE, Lenhoff AM (1998) J Colloid Interface Sci 203:218
Kim YP, Hong MY, Kim J, Oh E, Shon HK, Moon DW, Kim HS, Lee TG (2007) Anal Chem 79:1377
Ivanova EP, Wright JP, Pham DK, Brack N, Pigram P, Alekseeva YV, Demyashev GM, Nicolau DV (2006) Biomed Mater 1:24
Acknowledgments
This study was supported by the National Research Foundation of Korea (NRF) (K21003001810-11E0100-01510), and Basic Science Research Program through the NRF (2011-0022757), Pioneer Research Program for Converging Technology (20090081537), Advanced Biomass R&D Center (ABC) (ABC-2010-0029800) of Ministry of Education, Science and Technology (MEST) of Korea, and the Seoul R&D Program (10919).
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Young-Pil Kim and Sang Jin Park have contributed equally to this study.
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Kim, YP., Park, S.J., Lee, D. et al. Electrochemical glucose biosensor by electrostatic binding of PQQ-glucose dehydrogenase onto self-assembled monolayers on gold. J Appl Electrochem 42, 383–390 (2012). https://doi.org/10.1007/s10800-012-0409-1
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DOI: https://doi.org/10.1007/s10800-012-0409-1