International Ophthalmology

, Volume 33, Issue 3, pp 251–254 | Cite as

Is blood agar an alternative to sabouraud dextrose agar for the isolation of fungi in patients with mycotic keratitis

  • Ashok Kumar Reddy
  • Upputuri Brahmaiah
  • Nitesh Narayen
  • Ravi Kumar Reddy
  • Rupak Kumar Reddy
  • Meghraj Chitta
  • Srinivas Prasad
  • Rishi Swarup
  • Syed Maaz Mohiuddin
  • Madhukar Reddy
  • Murali K. Aasuri
  • B. S. R. Murthy
  • Milind Bhide
  • Sajid Ahmed
Original Paper

Abstract

To compare the blood agar (BA), sabouraud dextrose agar (SDA) and chocolate agar (CA) for the isolation of fungi in patients with mycotic keratitis. Corneal Scrapings of 229 patients with clinically diagnosed microbial keratitis were inoculated on BA, SDA, CA. The culture media were evaluated for the rate and time taken for the fungal growth. Seventy six of 229 patients had fungal keratitis. Fungus grew on BA in 60/76(78.9 %), on SDA in 76/76 (100 %), on CA in 40/76(52.6 %) patients. The fungi which grew on BA (60/76) also grown on SDA at the same time. The colony morphologies of different fungi were better on SDA than BA/CA. Among the different culture media, SDA is essential for the isolation fungi in patients with mycotic keratitis.

Keywords

Blood agar Sabouraud dextrose agar Mycotic keratitis Chocolate agar Culture media 

Notes

Conflict of interest

The authors declare that they have no conflicts of interest.

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Ashok Kumar Reddy
    • 1
  • Upputuri Brahmaiah
    • 2
  • Nitesh Narayen
    • 3
  • Ravi Kumar Reddy
    • 4
  • Rupak Kumar Reddy
    • 4
  • Meghraj Chitta
    • 4
  • Srinivas Prasad
    • 5
  • Rishi Swarup
    • 6
  • Syed Maaz Mohiuddin
    • 7
    • 13
  • Madhukar Reddy
    • 8
  • Murali K. Aasuri
    • 9
  • B. S. R. Murthy
    • 10
  • Milind Bhide
    • 11
  • Sajid Ahmed
    • 12
  1. 1.Department of MicrobiologyGHR Micro DiagnosticsHyderabadIndia
  2. 2.M.N.R Medical CollegeSangareddyIndia
  3. 3.Maxivision Eye CentreHyderabadIndia
  4. 4.Medivision Eye and Health Care CentreHyderabadIndia
  5. 5.Sarojini Devi Eye HospitalHyderabadIndia
  6. 6.Swarup Eye CentreHyderabadIndia
  7. 7.Eye Care HyderabadHyderabadIndia
  8. 8.Drishti Eye CentreHyderabadIndia
  9. 9.Sulochana Eye CentreHyderabadIndia
  10. 10.Vasan Eye CareHyderabadIndia
  11. 11.Hyderabad Eye CentreHyderabadIndia
  12. 12.Arvind Eye HospitalHyderabadIndia
  13. 13.Neo RetinaHyderabadIndia

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