Central nervous system evaluation of an ethanol extract of Bidens odorata Cav (Asteraceae) leaves, and its antinociceptive interaction with paracetamol and naproxen
- 19 Downloads
Bidens odorata Cav (Asteraceae) is a medicinal plant employed for the treatment of pain, anxiety, and depression. This study aimed to evaluate some neuropharmacological effects of an ethanol extract of B. odorata (BOE) and assess its antinociceptive interaction with naproxen and paracetamol.
Materials and methods
The following neuropharmacological effects were evaluated with the ethanolic extract of B. odorata leaves (BOE) (10–200 mg/kg p.o.): the strychnine-induced-convulsion assay (anticonvulsant effect), rotarod test (locomotor activity), tail suspension test (anti-depressant-like activity), cylinder exploratory test (anxiolytic-like actions), and pentobarbital-induced sleep test (sedative effect). The interaction of the BOE-paracetamol and BOE-naproxen combinations were evaluated with the acetic acid-induced writhing test. The ED50 value of each drug was estimated and the combinations of paracetamol and naproxen with BOE were calculated.
BOE (100–200 mg/kg) showed anti-convulsant activity by increasing the latency to occurrence of strychnine-induced convulsions, antidepressant-like effects by 28% and 33%, respectively, exerted anxiolytic actions (ED50 = 125 mg/kg), but did not affect motor locomotion. The pre-treatment with 2 mg/kg flumazenil or 20 mg/kg pentylenetetrazol partially reverted the anxiolytic activity shown by BOE alone. BOE (200 mg/kg) prolonged the duration of sleep with similar effect in comparison to clonazepam (1.5 mg/kg). The combinations of BOE-paracetamol (1:1) and BOE-naproxen (1:1) showed antinociceptive synergism.
BOE induces sedative and anticonvulsant effects. The anxiolytic actions shown by BOE are probably induced by the participation of the GABAergic system. BOE exerts antinociceptive synergistic interaction with paracetamol and naproxen probably by the participation of nitric oxide and ATP-sensitive K+ channels, respectively.
KeywordsBidens odorata Synergistic Antinociceptive Anticonvulsant Anxiolytic
This work was supported by the Consejo Nacional de Ciencia y Tecnologia (CONACyT; Ciencia Basica-CB-2015-01-257872; provided to JRZM). Special thanks to Jessica Mora (Universidad de Guanajuato) for her technical assistance.
All authors carried out chemical and pharmacological experiments. JRZM and AJAC supervised laboratory work, conceived the study, and wrote the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that there are no conflicts of interest.
- Alonso-Castro AJ, Domínguez F, Ruiz-Padilla AJ, Campos-Xolalpa N, Zapata-Morales JR, Carranza-Alvarez C, Maldonado-Miranda JJ (2017) Medicinal plants from North and Central America and the Caribbean considered toxic for humans: the other side of the coin. Evid Based Complement Altern Med. Article ID: 9439868Google Scholar
- Bello-González MA, Hernández-Muñoz S, Lara-Chávez MBN, Salgado-Garciglia R (2015) Plantas utiles de la comunidad indígena Nuevo San Juan Parangaritcutiro, Michoacan, Mexico. Polibotanica 39:175–215Google Scholar
- Beltrán-Villalobos KL, Déciga-Campos M, Aguilar-Mariscal H, González-Trujano ME, Martínez-Salazar MF, Ramírez-Cisneros MLÁ, Rios MY, López-Muñoz FJ (2017) Synergistic antinociceptive interaction of Syzygium aromaticum or Rosmarinus officinalis coadministered with ketorolac in rats. Biomed Pharmacother 94:858–864CrossRefGoogle Scholar
- Bernal-Morales B, Cueto-Escobedo J, Guillén-Ruiz G, Rodríguez-Landa JF, Contreras CM (2017) A fatty acids mixture reduces anxiety-like behaviors in infant rats mediated by GABAA receptors. Biomed Res Int Article ID: 8798546Google Scholar
- Jain NK, Kulkarni SK, Singh A (2001) Differential antinociceptive effect of cyclooxygenase inhibitors in acetic acid-induced chemonociception in mice. Analgesia 5(3–4):211–216Google Scholar
- Josabad Alonso-Castro A, Maldonado-Miranda JJ, Zarate-Martínez A, Jacobo-Salcedo MdR, Fernandez-Galicia C, Figueroa-Zuñiga LA, Rios-Reyes NA, de León-Rubio MA, Medellín-Castillo NA, Reyes-Munguia A, Mendez-Martinez R, Carranza-Álvarez C (2012) Medicinal plants used in the Huasteca Potosina, México. J Ethnopharmacol 143(1):292–298CrossRefGoogle Scholar
- Lehmann J, Hutchison A, McPherson SE, Mondadori C, Schmutz M, Sinton CM, Tsai C, Murphy DE, Steel DJ, Williams M, Cheney DL, Wood PL (1988) CGS 19755. A selective and competitive N-methyl-d-aspartate-type excitatory amino acid receptor antagonist. J Pharmacol Exp Ther 246(1):65–75PubMedGoogle Scholar
- NOM 062-ZOO-1999 Technical specifications for the production, care, and use of laboratory animalsGoogle Scholar
- Zapata-Morales JR, Alonso-Castro AJ, Domínguez F, Carranza-Álvarez C, Isiordia-Espinoza M, Hernández-Morales A, Solorio-Alvarado C (2017) The antinociceptive effects of a standardized ethanol extract of the Bidens odorata Cav (Asteraceae) leaves are mediated by ATP-sensitive K + channels. J Ethnopharmacol 207:30–33CrossRefGoogle Scholar