Anti-inflammatory and antinociceptive effects of an ethanol extract from Senna septemtrionalis
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Senna septemtrionalis (Viv.) H.S. Irwin & Barneby (Fabaceae) is a medicinal plant used as a folk remedy for inflammation and pain. The objective of this study was to evaluate the anti-inflammatory and antinociceptive actions of an ethanol extract of Senna septemtrionalis aerial parts (SSE). The in vitro anti-inflammatory effects of SSE were assessed using LPS-stimulated macrophages and the subsequent quantification of the levels of cytokines (IL-6, IL-1β, and TNF-α) with ELISA kits, nitric oxide (NO), and hydrogen peroxide (H2O2). The in vivo anti-inflammatory actions of SSE were evaluated with the TPA-induced ear oedema test and the carrageenan-induced paw oedema test. The antinociceptive actions of SSE (10–200 mg/kg p.o.) were assessed using three models: two chemical assays (formalin-induced orofacial pain and acetic acid-induced visceral pain) and one thermal assay (hot plate). SSE showed in vitro anti-inflammatory actions with IC50 values calculated as follows: 163.3 µg/ml (IL-6), 154.7 µg/ml (H2O2) and > 200 µg/ml (IL-1β, TNF-α, and NO). SSE showed also in vivo anti-inflammatory actions in the TPA test (40% of inhibition of ear oedema) and the carrageenan test (ED50 = 137.8 mg/kg p.o.). SSE induced antinociceptive activity in the formalin orofacial pain test (ED50 = 80.1 mg/kg) and the acetic acid-induced writhing test (ED50 = 110 mg/kg). SSE showed no antinociceptive actions in the hot plate assay. The pre-treatment with glibenclamide abolished the antinociceptive action shown by SSE alone. Overall, SSE exerted in vitro and in vivo anti-inflammatory actions, and in vivo antinociceptive effects by the possible involvement of ATP-sensitive K + channels.
KeywordsSenna septemtrionalis Antinociceptive Anti-inflammatory Medicinal plant
This work was partially supported by the Directorate for Research Support and Postgraduate Programs (DAIP) at University of Guanajuato (DAIP, CIIC 67/2019, provided to AJAC). An experimental section of this work was supported by the program SEP-PRODEP (Apoyo a la Incorporación de Nuevos PTC, Grant Number: UDG-PTC-1438 Number 511-6/18/9169, provided to MAIE).
Compliance with ethical standards
Conflict of interest
The authors have no conflict of interest to declare.
- Aguilar A, Argueta A, Cano L (1994) Flora medicinal indígena de México. Instituto Nacional Indigenista México City, MexicoGoogle Scholar
- Allkin B (2017) Useful plants—medicines: at least 28,187 plant species are currently recorded as being of medicinal use. In: Willis KJ (ed) State of the world’s plants 2017. Royal Botanic Gardens, Kew, London (UK)Google Scholar
- Alonso-Castro AJ, Alba-Betancourt C, Yáñez-Barrientos E, Luna-Rocha C, Páramo-Castillo AS, Aragón-Martínez OH, Zapata-Morales JR, Cruz-Jiménez G, Gasca-Martínez D, González-Ibarra AA, Álvarez-Camacho DA, Devezé-Álvarez MA (2019) Diuretic activity and neuropharmacological effects of an ethanol extract from Senna septemtrionalis (Viv.) H.S. Irwin & Barneby (Fabaceae). J Ethnopharmacol 239:111923CrossRefGoogle Scholar
- Barreras-Espinoza I, Soto-Zambrano JA, Serafín-Higuera N, Zapata-Morales R, Alonso-Castro Á, Bologna-Molina R, Granados-Soto V, Isiordia-Espinoza MA (2017) The antinociceptive effect of a tapentadol-ketorolac combination in a mouse model of trigeminal pain is mediated by opioid receptors and ATP-sensitive K + channels. Drug Dev Res 78(1):63–70CrossRefGoogle Scholar
- do Nascimento JET, de Morais SM, de Lisboa DS, de Oliveira Sousa M, Santos SAAR, Magalhães FEA, Campos AR (2018) The orofacial antinociceptive effect of Kaempferol-3-O-rutinoside, isolated from the plant Ouratea fieldingiana, on adult zebrafish (Danio rerio). Biomed Pharmacother 107:1030–1036CrossRefGoogle Scholar
- Siqueira-Lima PS, Araújo AA, Lucchese AM, Quintans JS, Menezes PP, Alves PB, de Lucca Júnior W, Santos MR, Bonjardim LR, Quintans-Júnior LJ (2014) β-cyclodextrin complex containing Lippia grata leaf essential oil reduces orofacial nociception in mice—evidence of possible involvement of descending inhibitory pain modulation pathway. Basic Clin Pharmacol Toxicol 114(2):188–196CrossRefGoogle Scholar