, Volume 27, Issue 6, pp 1255–1263 | Cite as

Pterodontic acid isolated from Laggera pterodonta suppressed RIG-I/NF-KB/STAT1/Type I interferon and programmed death-ligand 1/2 activation induced by influenza A virus in vitro

  • Yutao Wang
  • Zhiqi Zeng
  • Qiaolian Chen
  • Wen Yan
  • Yunbo Chen
  • Xuanzi Xia
  • Wenjun SongEmail author
  • Xinhua WangEmail author
Original Article


Influenza viruses can bring about acute respiratory diseases and are a potential hazard to human health. Antiviral drugs are the main ways to control the influenza virus infection except the vaccine. In this study, the immune regulation activity of pterodontic acid isolated from Laggera pterodonta induced by influenza A virus in vitro was evaluated. In studies on anti-influenza activity, our results showed that it maybe target the influenza protein of polymerase basic 1 (PB1), polymerase basic 2 (PB2), polymerase acid (PA), nuclear protein (NP), non-structural protein (NS), and matrix protein (M) but not hemagglutinin (HA) and neuraminidase (NA). In studies on immune regulation, our results demonstrated that pterodontic acid can inhibit the Retinoic acid inducible gene-I (RIG-I) expression in mRNA and protein level at 100 μg/ml, then further to clarify its action on the signalling pathway, The results indicated that pterodontic acid can inhibit the Tumor Necrosis Factor-related Apoptosis-inducing Ligand/Fas Ligand (TRAIL/Fasl) expression in mRNA level at 100 μg/ml; the cleaved caspase 3/7, p-NF-KB, and p-ERK were all suppressed in protein level by pterodontic acid at 100 μg/ml. This confirmed its mechanism that restrained the nuclear export of viral RNPs. The interferon system was also affected, the STAT1, IFN-α, IFN-β expression were also inhibited by pterodontic acid at 25–100 μg/ml and also, the important programmed death-ligand of PD-L1 and PD-L2 was inhibited at 50–100 μg/ml. The mechanisms of pterodontic acid against influenza virus infection may be a cascade inhibition and it has the anti-inflammatory activity, which has no side effect, and can be as a supplement drug in clinical influenza virus infection.


Pterodontic acid Influenza virus Immunomodulation 



This work was supported by the National Natural Science Foundation of China (Grant no. U1502226), the Engineering Technology Research Center (Development) of Guangdong general universities (Grant no. GCZX-A1408), and Youth Innovative Talents project of Department of Education of Guangdong Province (Grant no. 2017KQNCX169).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Yutao Wang
    • 1
  • Zhiqi Zeng
    • 1
  • Qiaolian Chen
    • 1
  • Wen Yan
    • 2
  • Yunbo Chen
    • 2
  • Xuanzi Xia
    • 1
  • Wenjun Song
    • 3
    Email author
  • Xinhua Wang
    • 1
    • 3
    Email author
  1. 1.State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory HealthFirst Affiliated Hospital of Guangzhou Medical UniversityGuangzhouPeople’s Republic of China
  2. 2.Institute of Tropical MedicineGuangzhou University of Chinese MedicineGuangzhouPeople’s Republic of China
  3. 3.State Key Laboratory of Respiratory Disease, Institute of Chinese Integrative Medicine, First Affiliated HospitalGuangzhou Medical UniversityGuangzhouPeople’s Republic of China

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