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Topical application of Mentha piperita essential oil accelerates wound healing in infected mice model

  • Mohammad Modarresi
  • Mohammad-Reza Farahpour
  • Behzad Baradaran
Original Article

Abstract

This study was conducted to evaluate the effects of the prepared ointments from Mentha piperita essential oil (M. piperita) on wound healing in the infected mice models. Each circular full-thickness wound was inoculated with 25 × 107 units of Staphylococcus aureus and Pseudomonas aeruginosa bacteria strains. The tissue bacterial count, histological analyses and expression levels of IL-10, TNF-α, TGF-β1, IL-1β, CCL2, CXCL1, VEGF and FGF-2 were assessed to identify the different doses of M. piperita on wound healing. Total tissue bacterial count, edema and inflammation level were declined, but the migration of fibroblasts, collagen synthesis and re-epithelization were increased in treated animals with M. piperita. The expression levels of CCL2, CXCL1, IL-1β, TGF-β1 and IL-10 genes were up-regulated in the M. piperita-treated animals compared to the control group. While the expression of TNF-α, VEGF and FGF-2 was down-regulated in comparison to the control group. This study indicated that M. piperita can be used for treatment of the infected wound.

Keywords

Mentha piperita Infectious wound Pro-inflammatory cytokine Collagen synthesis Re-epithelization 

Notes

Acknowledgement

This study was extracted from DVM thesis of Mr Mohammad Moddaresi in Veterinary Faculty of Islamic Azad University, Urmia Branch. The authors are grateful to Dr. Farahnaz Tahery for ointment formulation and Dr. Sheryl Thomas for the native language edition.

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Mohammad Modarresi
    • 1
  • Mohammad-Reza Farahpour
    • 2
  • Behzad Baradaran
    • 3
  1. 1.Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia BranchIslamic Azad UniversityUrmiaIran
  2. 2.Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia BranchIslamic Azad UniversityUrmiaIran
  3. 3.Immunology Research CenterTabriz University of Medical SciencesTabrizIran

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