Topical application of Mentha piperita essential oil accelerates wound healing in infected mice model
This study was conducted to evaluate the effects of the prepared ointments from Mentha piperita essential oil (M. piperita) on wound healing in the infected mice models. Each circular full-thickness wound was inoculated with 25 × 107 units of Staphylococcus aureus and Pseudomonas aeruginosa bacteria strains. The tissue bacterial count, histological analyses and expression levels of IL-10, TNF-α, TGF-β1, IL-1β, CCL2, CXCL1, VEGF and FGF-2 were assessed to identify the different doses of M. piperita on wound healing. Total tissue bacterial count, edema and inflammation level were declined, but the migration of fibroblasts, collagen synthesis and re-epithelization were increased in treated animals with M. piperita. The expression levels of CCL2, CXCL1, IL-1β, TGF-β1 and IL-10 genes were up-regulated in the M. piperita-treated animals compared to the control group. While the expression of TNF-α, VEGF and FGF-2 was down-regulated in comparison to the control group. This study indicated that M. piperita can be used for treatment of the infected wound.
KeywordsMentha piperita Infectious wound Pro-inflammatory cytokine Collagen synthesis Re-epithelization
This study was extracted from DVM thesis of Mr Mohammad Moddaresi in Veterinary Faculty of Islamic Azad University, Urmia Branch. The authors are grateful to Dr. Farahnaz Tahery for ointment formulation and Dr. Sheryl Thomas for the native language edition.
Compliance with ethical standards
Conflict of interest
The authors declare that there are no conflicts of interest.
- Bielefeld KA, Amini-Nik S, Alman BA (2013) Cutaneous wound healing: recruiting developmental pathways for regeneration. Cell Mol Life Sci 6:1–23Google Scholar
- Çetin B, Kaya Y, Çakir A, Özer H, Aksakal Ö, Mete E (2016) Antimicrobial activities of essential oils and hexane extracts of two Turkish spice plants, cymbocarpum erythraeum (DC.) Boiss. and Echinophora tenuifolia L. against foodborne microorganisms. Rec Nat Prod 10(4):426–435Google Scholar
- Hozzeina WN, Badrc G, Al Ghamdid A, Sayede A, Al-Wailif NS, Garraudg O (2015) Topical application of propolis enhances cutaneous wound healing by promoting TGF-Beta/smad-mediated collagen production in a streptozotocin-induced type I diabetic mouse model. Cell Physiol Biochem 37:940–954CrossRefGoogle Scholar
- Jianu C, Pop G, Gruia AT, Horhat FG (2013) Chemical composition and antimicrobial activity of essential oils of lavender (Lavandula angustifolia) and lavandin (Lavandula × intermedia) grown in Western Romania. Int J Agric Biol 15(4):772–776Google Scholar
- Oryan A, Moshiri A (2011) A long term study on the role of exogenous human recombinant basic fibroblast growth factor on the superficial digital flexor tendon healing in rabbits. J Musculoskelet Neuron Interact 11(2):185–195Google Scholar