Brazilian green propolis hydroalcoholic extract reduces colon damages caused by dextran sulfate sodium-induced colitis in mice
This study investigated the effects of Brazilian green propolis hydroalcoholic extract (BPE) in 3% w/v dextran sodium sulfate (DSS)-induced colitis in mice. The effects of BPE (3, 30 and 300 mg/kg, p.o, by 7 days) on the morphological (colon length and colon weight), clinical (disease activity index and weight loss), microscopic (histological score and mucin levels) and biochemical parameters were determined. The effects of BPE (300 mg/kg, p.o) in the gastrointestinal transit of mice were also evaluated. As expected, the DSS ingestion damaged the colonic tissue, lowered the body weight, decreased the mucin levels, increased MPO activity, reduced SOD activity and GSH amount. In contrast, the treatment with BPE (300 mg/kg) significantly reduced macroscopic colonic injury and the mucosal damage in colon on histopathological examination and reversed the decrease in mucin levels induced by DSS. It also significantly normalized the SOD activity and the levels of GSH, but did not elicit any effect on MPO activity in the colon. In addition, BPE did not change the gastric emptying or the intestinal transit rate of mice. Together, these results suggested that BPE reduced the signs of DSS-induced colitis in mice through maintenance of intestinal mucin barrier and favoring intestinal antioxidant defenses.
KeywordsColitis DSS Brazilian green propolis
The authors are thankful to the São Paulo Research Foundation for financial support, Grant number 2017/04138-8. In addition, we are grateful to Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Universidade do Vale do Itajaí (UNIVALI) for their financial support. Moreover, RCMVAFS is grateful for the postdoctoral scholarship from PNPD/CAPES.
- Bezerra BG, De Menezes, De Souza L, Dos Santos AS, De Almeida GK, Souza MT, Santos SL, Aparecido CE, Dos Santos LB, De Souza AAA, Cardoso JC, Gomes SV, Gomes MZ, Júnior ARL (2017) Hydroalcoholic extract of Brazilian red propolis exerts protective effects on acetic acid-induced ulcerative colitis in a rodent model. Biomed Pharmacother 85:687–696CrossRefGoogle Scholar
- Chassaing B, Aitken JD, Malleshappa M, Vijay-Kumar M (2014) Dextran sulfate sodium (DSS)-induced colitis in mice. Curr Protoc Immunol 104:15–25Google Scholar
- Da Silva LM, Farias JA, Boeing T, Somensi LB, Beber AP, Cury BJ, Santin JR, de Faloni AS (2016) Hydroalcoholic extract from inflorescences of Achyrocline satureioides (Compositae) ameliorates dextran sulphate sodium-induced colitis in mice by attenuation in the production of inflammatory cytokines and oxidative mediators. Evi Bas Com Art Med 2016:1–15Google Scholar
- De Moura SAL, Ferreira MAND, Andrade SP, Reis MLC, De Noviello M, Cara DC (2011) Brazilian green propolis inhibits inflammatory angiogenesis in a murine sponge model. Evid Based Complement Alternat Med 2011:1–7Google Scholar
- Doi K, Fujioka M, Sokuza Y, Ohnishi M, Gi M, Takeshita M, Kumada K, Kakehashi A, Wanibuchi H (2017) Chemopreventive action by ethanol-extracted Brazilian green propolis on post-initiation phase of inflammation-associated rat colon tumorigenesis. In Vivo 31(2):187–198CrossRefPubMedPubMedCentralGoogle Scholar
- Freire AC, Podczeck F, Sousa J, Veiga F (2006) Liberação específica de fármacos para administração no cólon por via oral. I-O cólon como local de liberação de fármacos. Brazil J Pharma Sci 42:319–335Google Scholar
- Laroui H, Ingersoll SA, Liu HC, Baker MT, Ayyadurai S, Charania MA, Laroui F, Yan Yutao, Sitaraman SV, Merlin D (2012) Dextran sodium sulfate (DSS) induces colitis in mice by forming nano-lipocomplexes with medium-chain-length fatty acids in the colon. PLoS ONE 7:e32084CrossRefPubMedPubMedCentralGoogle Scholar
- Pereira C, Grácio D, Teixeira JP, Magro F (2015) Oxidative stress and DNA damage: implications in inflammatory bowel disease. Inflamm Bowel Dis 2015(21):2403–2417Google Scholar