, Volume 26, Issue 3, pp 829–838 | Cite as

Effects of glutamine, taurine and their association on inflammatory pathway markers in macrophages

  • Talita Sartori
  • Guilherme Galvão dos Santos
  • Amanda Nogueira-Pedro
  • Edson Makiyama
  • Marcelo Macedo Rogero
  • Primavera Borelli
  • Ricardo Ambrósio FockEmail author
Original Article


The immune system is essential for the control and elimination of infections, and macrophages are cells that act as important players in orchestrating the various parts of the inflammatory/immune response. Amino acids play important role in mediating functionality of the inflammatory response, especially mediating macrophages functions and cytokines production. We investigated the influence of glutamine, taurine and their association on the modulation of inflammatory pathway markers in macrophages. The RAW 264.7 macrophage cell line was cultivated in the presence of glutamine and taurine and proliferation rates, cell viability, cell cycle phases, IL-1α, IL-6, IL-10 and TNF-α as well as H2O2 production and the expression of the transcription factor, NFκB, and its inhibitor, IκBα, were evaluated. Our results showed an increase in viable cells and increased proliferation rates of cells treated with glutamine concentrations over 2 mM, as well as cells treated with both glutamine and taurine. The cell cycle showed a higher percentage of cells in the phases S, G2 and M when they were treated with 2 or 10 mM glutamine, or with glutamine and taurine in cells stimulated with lipopolysaccharide. The pNFκB/NFκB showed reduced ratio expression when cells were treated with 10 mM of glutamine or with glutamine in association with taurine. These conditions also resulted in reduced TNF-α, IL-1α and H2O2 production, and higher production of IL-10. These findings demonstrate that glutamine and taurine are able to modulate macrophages inflammatory pathways, and that taurine can potentiate the effects of glutamine, illustrating their immunomodulatory properties.


Glutamine Taurine Macrophages NFκB Cytokines 



This work was supported by Fundação de Amparo a Pesquisa do Estado de São Paulo FAPESP (Grant no. 2016/16463-8). Rogero MM, Borelli P and Fock RA are fellows of the Conselho Nacional de Pesquisa e Tecnologia (CNPq).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  • Talita Sartori
    • 1
  • Guilherme Galvão dos Santos
    • 1
  • Amanda Nogueira-Pedro
    • 1
  • Edson Makiyama
    • 1
  • Marcelo Macedo Rogero
    • 2
  • Primavera Borelli
    • 1
  • Ricardo Ambrósio Fock
    • 1
    Email author
  1. 1.Department of Clinical and Toxicological Analysis, School of Pharmaceutical SciencesUniversity of São PauloSão PauloBrazil
  2. 2.Department of Nutrition, School of Public HealthUniversity of São PauloSão PauloBrazil

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