, Volume 25, Issue 6, pp 609–619 | Cite as

Immunomodulatory potential of nanocurcumin-based formulation

  • Mahendra Kumar Trivedi
  • Sambhu Charan Mondal
  • Mayank Gangwar
  • Snehasis JanaEmail author
Original Article


Vitamins, minerals, and nanocurcumin play a substantial role in various nutraceutical/pharmaceutical formulations that are widely used in therapeutics, cosmetics, and dietary supplements. The current study aimed to investigate the comparative in vitro immunomodulatory effect of a novel nanocurcumin-based formulation with curcumin in LPS-induced cytokine expression, NK cells’ activity, and phagocytosis. The proinflammatory cytokines (TNF-α, IL-1β, and MIP-1α) and NK cells’ activity were measured in cell supernatants using ELISA assay; however, phagocytosis activity was performed using colorimetric analysis. The chemical characterization of novel nanocurcumin-based formulation using LC–MS (R t 19.02 min) and mass spectra analysis (m/z 369.04) confirmed the presence of the curcumin in highest peak concentration. MTT assay in three tested cell-lines showed that the formulation was found non-toxic at all the tested concentrations. The expression of TNF-α, IL-1β, and MIP-1α in splenocytes was significantly (p ≤ 0.001) inhibited. Besides, the NK cells’ activity and phagocytosis (macrophage) were increased significantly (p ≤ 0.001). Overall, the promising results of this study indicated the significant immunomodulatory effect of nanocurcumin-based formulation compared to the curcumin, which could be used against various inflammatory disorders such as allergy, asthma, autoimmune diseases, coeliac disease, inflammatory bowel disease, etc.

Graphical Abstract


Nanocurcumin Immunomodulation Pro-inflammatory cytokines Natural killer cells Phagocytosis Liquid chromatography-mass spectrometry 



The authors are grateful to the support of Trivedi Science, Trivedi Global, Inc., and Trivedi Master Wellness throughout the work. Authors also thankful to Dabur Research Foundation (DRF) for conducting the experiment.

Compliance with ethical standards

Conflict of interest

The authors report no conflicts of interest.


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Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  • Mahendra Kumar Trivedi
    • 1
  • Sambhu Charan Mondal
    • 2
  • Mayank Gangwar
    • 2
  • Snehasis Jana
    • 2
    Email author
  1. 1.Trivedi Global, Inc.HendersonUSA
  2. 2.Trivedi Science Research Laboratory Pvt. Ltd.BhopalIndia

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