, Volume 23, Issue 6, pp 291–305 | Cite as

Neuroprotective mechanism of losartan and its interaction with nimesulide against chronic fatigue stress

  • Anil KumarEmail author
  • Barinder Singh
  • Jitendriya Mishra
  • Sangeeta Pilkhwal Sah
  • Raghavender Pottabathini
Research Article


Potential role of angiotensin-II and cyclooxygenase have been suggested in the pathophysiology of chronic fatigue stress. The present study has been designed to evaluate the neuroprotective effect of losartan and its interaction with nimesulide against chronic fatigue stress and related complications in mice. In the present study, male Laca mice (20–30 g) were subjected to running wheel activity test session (RWATS) for 6 min daily for 21 days. Losartan, nimesulide and their combinations were administered daily for 21 days, 45 min before being subjected to RWATS. Various behavioral and biochemical and neuroinflammatory mediators were assessed subsequently. 21 days RWATS treatment significantly decreased number of wheel rotations/6 min indicating fatigue stress like behaviors as compared to naive group. 21 days treatment with losartan (10 and 20 mg/kg, ip), nimesulide (5 and 10 mg/kg, po) and their combinations significantly improved behavior [increased number of wheel rotations, reversal of post-exercise fatigue, locomotor activity, antianxiety-like behavior (number of entries, latency to enter and time spent in mirror chamber), and memory performance (transfer latency in plus-maze performance task)], biochemical parameters (reduced serum corticosterone, brain lipid peroxidation, nitrite concentration, acetylcholinesterase activity, restored reduced glutathione levels and catalase activity) as compared to RWATS control. Besides, TNF-α, CRP levels were significantly attenuated by these drugs and their combinations as compared to control. The present study highlights the role of cyclooxygenase modulation in the neuroprotective effect of losartan against chronic fatigue stress-induced behavioral, biochemical and cellular alterations in mice.


Fatigue Stress Oxidative damage Losartan RWATS 



Authors gratefully acknowledge the financial support of University Grants Commission (New Delhi) for carrying out this research work.

Compliance with ethical standard heading

Conflict of interest

Authors declare that they do not have any competing financial interests. There is no conflict of interest between the authors to declare.


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Copyright information

© Springer Basel 2015

Authors and Affiliations

  • Anil Kumar
    • 1
    Email author
  • Barinder Singh
    • 1
  • Jitendriya Mishra
    • 1
  • Sangeeta Pilkhwal Sah
    • 1
  • Raghavender Pottabathini
    • 1
  1. 1.Pharmacology Division, University Institute of Pharmaceutical Sciences, UGC-Center of Advanced Study (UGC-CAS)Panjab UniversityChandigarhIndia

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