Advertisement

Inflammopharmacology

, Volume 20, Issue 6, pp 315–322 | Cite as

A novel antimicrobial lectin from Eugenia malaccensis that stimulates cutaneous healing in mice model

  • V. P. Brustein
  • F. V. Souza-Araújo
  • A. F. M. Vaz
  • R. V. S. Araújo
  • P. M. G. Paiva
  • L. C. B. B. Coelho
  • A. M. A. Carneiro-Leão
  • J. A. Teixeira
  • M. G. Carneiro-da-Cunha
  • M. T. S. Correia
Research Article

Abstract

Objective

The present work reports the purification and partial characterization of an antibacterial lectin (EmaL) obtained from Eugenia malaccensis seeds as well as the evaluation of its effect in the daily topical treatment of repairing process of cutaneous wounds in mice.

Materials and methods

The cutaneous wound was produced by the incision of the skin and use of lectin in the treatment of mice cutaneous wounds was evaluated. Surgical wounds were treated daily with a topical administration of EmaL and parameters such as edema, hyperemia, scab, granulation and scar tissues as well as contraction of wounds were analyzed.

Results

A novel lectin, with a molecular mass of 14 kDa, was isolated from E. malaccensis using affinity chromatography. The lectin (EmaL) agglutinated glutaraldehyde-treated rabbit and human erythrocytes; the lectin-induced rabbit erythrocyte agglutination was inhibited by glucose, casein, ovalbumin and fetuin. Also, Emal was very effective in the inhibition of bacterial growth, with the best inhibition results obtained for Staphylococcus aureus. Inflammatory signals such as edema and hyperemia were statistically less intense when EmaL was applied compared to the control. The histopathological analysis showed that the treated injured tissue presented reepithelialization (complete or partial) and areas of transition more evidenced than those of the control group, especially due to well organized pattern of collagen fibers presented in the granulation fibrous tissue.

Conclusion

Presented results are a preliminary indication of the pharmacological interest in using EmaL as antimicrobial agent and in the repairing process of cutaneous wounds.

Keywords

Eugenia malaccensis Lectin Antibacterial Cutaneous wound healing 

Notes

Acknowledgments

This paper was financially supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), FACEPE and CAPES, Brazil. The authors are deeply grateful for the technical assistance of Maria Barbosa Reis da Silva and João Antonio Virgínio and Alfa/VALNATURA Project.

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Agrawal BBL, Goldstein IJ (1967) Protein carbohydrate interaction. VI. Isolation of concanavalin A by specific adsorption on cross-linked dextran gels. Biochim Biophys Acta 147:262–271PubMedCrossRefGoogle Scholar
  2. Alencar NMN, Assreuy AMS, Alencar VBM et al (2003) The galactose-binding lectin from Vatairea macrocarpa seeds induces in vivo neutrophil migration by indirect mechanism. Int J Biochem Cell Biol 35:1674–1681PubMedCrossRefGoogle Scholar
  3. Alencar VBM, Alencar MNN, Assreuy AMS et al (2005) Pro-inflammatory effect of Arum maculatum lectin and role of resident cells. Int J Biochem Cell Biol 37:1805–1814PubMedCrossRefGoogle Scholar
  4. Assreuy AM, Alencar NMN, Cavada BS et al (2003) Porcine spermadhesin PSP-I/PSP-II stimulates macrophages to release a neutrophil chemotactic substance: modulation by mast cells. Biol Reprod 68:1836–1841PubMedCrossRefGoogle Scholar
  5. Barondes SH (1988) Bifunctional properties of lectins: lectins redefined. Trends Biochem Sci 13:480–482PubMedCrossRefGoogle Scholar
  6. Bauer AW, Kirby WMM, Sherres JC et al (1966) Antibiotic susceptibility testing by a standardised single disc method. Am J Clin Pathol 45:493–496PubMedGoogle Scholar
  7. Branski RC, Rosen CA, Verdoline K, Hebda PA (2005) Biochemical markers associated with acute vocal fold wound healing: a rabbit model. J Voice 19:283–289PubMedCrossRefGoogle Scholar
  8. Clark RAF (2001) Fibrin and wound healing. Ann NY Acad Sci 936:355–367PubMedCrossRefGoogle Scholar
  9. Correia MTS, Coelho LCBB (1995) Purification of a glucose/mannose specific lectin, isoform 1, from seeds of Cratylia mollis Mart. (Camaratu bean). Appl Biochem Biotechnol 55:261–273PubMedCrossRefGoogle Scholar
  10. Courvalin P, Goldstein F, Philippon A, Sirot J (1985) L’antibiogramme MPC. Vigot, Bruxelles, pp 191–195Google Scholar
  11. De Melo CM, Porto CS, Melo-Júnior MR et al (2011) Healing activity induced by Cramoll 1,4 lectin in healthy and immunocompromised mice. Int J Pharm 15:113–119CrossRefGoogle Scholar
  12. Dubois B, Peumans WJ, Van Damme EJM et al (1998) Regulation of gelatinase B (MMP-9) in leukocytes by plant lectins. FEBS Lett 427:275–278PubMedCrossRefGoogle Scholar
  13. Gaidamashvili M, Standen JV (2002) Interaction of lectin-like proteins of South African medicinal plants with Staphylococcus aureus and Bacillus subtilis. J Ethnopharmacol 80:131–135PubMedCrossRefGoogle Scholar
  14. Hall LW, Clarke KW (1991) Veterinary anaesthesia, 9th edn. Ballière Tindall, LondonGoogle Scholar
  15. Inngjerdinger K, Nergard CS, Diallo D et al (2004) An ethnopharmacological survey of plants used for wound healing in Dongoland, Mali. West Africa J Ethnopharm 92:233–244CrossRefGoogle Scholar
  16. Konozy EHE, Bernardes ES, Rosa C et al (2003) Isolation, purification, and physicochemical characterization of a d−-galactose-binding lectin from seeds of Erythrina speciosa. Arch Biochem Biophys 410:222–229PubMedCrossRefGoogle Scholar
  17. Laemmli UK (1970) Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227:680–685PubMedCrossRefGoogle Scholar
  18. Laurens N, Koolwijk P, De Maat MPM (2006) Fibrin structure and wound healing. J Thromb Haem 4:932–939CrossRefGoogle Scholar
  19. Locher CP, Burch MT, Mower HF et al (1995) Anti-microbial activity and anti-complement activity of extracts obtained from selected Hawaiian medicinal plants. J Ethnopharmacol 49:23–32PubMedCrossRefGoogle Scholar
  20. Locher CP, Witvrouw M, De Bethune MP et al (1996) Antiviral activity of Hawaiian medicinal plants against human immunodeficiency virus type-1 (HIV-1). Phytomedicine 2:259–264CrossRefGoogle Scholar
  21. Lowry OH, Rosebrough NJ, Farr AL et al (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275PubMedGoogle Scholar
  22. Maciel EVM, Araujo-Filho VS, Nakazawa M et al (2004) Mitogenic activity of Cratylia mollis lectin on human lymphocytes. Biologicals 32:57–60PubMedCrossRefGoogle Scholar
  23. Merril CR, Goldman D, Sedman AS et al (1981) Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science 211:1437–1438PubMedCrossRefGoogle Scholar
  24. Morton J, Malay A (1987) Fruits of warm climates. Julia F. Morton, Miami, pp 378–381Google Scholar
  25. Nagase H, Visse R, Murphy G (2006) Structure and function of matrix metalloproteinases and TIMPs. Cardiovasc Res 69:562–573PubMedCrossRefGoogle Scholar
  26. Peumans WJ, Van Damme EJM (1998) Plant lectins versatile proteins with important perspectives in biotechnology. Biotechnol Genet Eng Rev 15:199–228Google Scholar
  27. Pistole TG (1981) Interaction of bacteria and fungi with lectins and lectin-like substances. Annu Rev Microbiol 35:85–112PubMedCrossRefGoogle Scholar
  28. Ramsey D (1995) Effects of three occlusive dressing materials on healing of full-thickness skin wounds in dogs. Am J Vet Res 56:941–949PubMedGoogle Scholar
  29. Ratanapo S, Ngamjunyaporn W, Chulavatnatol M (2001) Interaction of a mulberry leaf lectin with a phytopathogenic bacterium, P. syringae pv mori. Plant Sci 160:739–744PubMedCrossRefGoogle Scholar
  30. Sell AM, Costa CP (2003) Effects of plant lectins on in vitro fibroblast proliferation. Braz Arch Biol Technol 46:349–354CrossRefGoogle Scholar
  31. Sharon N, Lis H (1990) Legum lectins, a large family of homologous proteins. Faseb J 4:3198–3208PubMedGoogle Scholar
  32. Sharon N, Lis H (2004) History of lectins: from hemagglutinins to biological recognition molecules. Glycobiology 14:53–62CrossRefGoogle Scholar
  33. Singer AJ, Clark RAF (1999) Cutaneous wound healing. N Engl J Med 341:738–746PubMedCrossRefGoogle Scholar
  34. Singer AJ, Mcclain A (2002) Persistent wound infection delays epidermal maturation and increases scarring in thermal burns. Wound Repair Regen 10:372–377PubMedCrossRefGoogle Scholar
  35. Slifkin M, Doyle RJ (1990) Lectins and their application in clinical microbiology. Clin Microbiol Rev 3:197–218PubMedGoogle Scholar
  36. Spector M (2001) Biomaterials. In: Achauer B, Eriksson E, Guyuron B (eds) Plastic surgery, indications, operations, outcomes. Mosby Year Book, pp 239–259Google Scholar
  37. Whistler Polynesian Herbal Medicine (1992) Everbest Publishing Co., Hong KongGoogle Scholar

Copyright information

© Springer Basel AG 2012

Authors and Affiliations

  • V. P. Brustein
    • 1
  • F. V. Souza-Araújo
    • 1
  • A. F. M. Vaz
    • 1
  • R. V. S. Araújo
    • 2
  • P. M. G. Paiva
    • 1
  • L. C. B. B. Coelho
    • 1
  • A. M. A. Carneiro-Leão
    • 3
  • J. A. Teixeira
    • 4
  • M. G. Carneiro-da-Cunha
    • 1
    • 2
  • M. T. S. Correia
    • 1
  1. 1.Departament of BiochemistryUniversidade Federal de Pernambuco (UFPE)RecifeBrazil
  2. 2.Laboratory of Immunopathology Keizo AsamiUniversidade Federal de PernambucoRecifeBrazil
  3. 3.Department of Animal Morphology and PhysiologyUniversidade Federal Rural de PernambucoRecifeBrazil
  4. 4.Institute for Biotechnology and Bioengineering, Centre for Biological EngineeringUniversidade do MinhoBragaPortugal

Personalised recommendations