Inhibition of pro-inflammatory mediators: role of Bacopa monniera (L.) Wettst
Bacopa monniera (L.) Wettst is a renowned plant in the Ayurvedic system of medicine. The present study seeks to identify the anti-inflammatory activity of two fractions from the methanolic extract of Bacopa, viz. the triterpenoid and bacoside-enriched fractions. The ability of these two fractions to inhibit the production of pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and interleukin-6 was tested using lipopolysaccharide (LPS)-activated peripheral blood mononuclear cells and peritoneal exudate cells in vitro. We found that triterpenoid and bacoside-enriched fractions significantly inhibited LPS-activated TNF-α, IL-6 and nitrite production in mononuclear cells. Significant antioxidant activity was exhibited by the bacoside enriched fraction compared to the triterpenoid fraction. Carrageenan-induced hind paw oedema assay revealed that triterpenoid and bacoside-enriched fractions exerted anti-oedematogenic effect, while in the arthritis model only the triterpenoid fraction exerted an anti-arthritic potential. The present study provides an insight into the ability of Bacopa monniera to inhibit inflammation through modulation of pro-inflammatory mediator release.
KeywordsInflammation Tumor necrosis factor-α Interleukin Oedema Bacopa monniera Arthritis Nitric oxide Bacoside Triterpenoid
This work was supported by the Kerala State Council for Science, Technology and Environment, Sasthra Bhavan, Thiruvananthapuram, Kerala, India. Standardized Bacopa extract was obtained as a gift from Natural Products Pvt Ltd., Bangalore, India.
Conflict of interest statement
The authors declare that there were no conflicts of interest.
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