Heavy Metals Effect on Cyanobacteria Synechocystis aquatilis Study Using Absorption, Fluorescence, Flow Cytometry, and Photothermal Measurements
The toxic effect of six heavy metals on cyanobacteria Synechocystis aquatilis was studied by absorption, fluorescence, flow cytometry, and photothermal measurements. This study indicates that at the concentration used, the cyanobacteria are more sensitive to silver, copper, and mercury than to cadmium, lead, and zinc metals. Disregarding the decrease in the yields of the related radiative processes caused by photochemical processes and/or damage to phycobilisomes, no changes were detected in the efficiency of thermal deactivation processes within a few microseconds, which can indicate the lack of disturbances in the photosynthetic light reaction and the lack of damage to the photosystem caused by the heavy metal ions in the concentrations used. The results demonstrate that the relative values of fluorescence yield as well as promptly generated heat calculated for the metal-affected and unaffected (reference) bacteria are sensitive indicators of environmental pollution with heavy metal ions, whereas the complementary methods proposed could be used as a noninvasive and fast procedure for in vivo assessment of their toxicity.
KeywordsChlorophyll Cyanobacteria Heavy metal Optical and photothermal spectroscopy Synechocystis aquatilis
This study was supported by Poznan University of Technology.
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- 3.M.K. Joshi, P. Mohanty, in Chlorophyll a Fluorescence: A Signature of Photosynthesis, ed. by G.C. Papageorgiou, G.C. Govindjee. (Springer, Dordrecht, 2004), pp. 637–661Google Scholar
- 5.Chaloub R.M., de Magalhaes C.C.P., dos Santos C.P.: J. Physiol. 41, 1162 (2005)Google Scholar
- 12.W.F.J. Vermaas, in Encyclopedia of Life Sciences (Macmillian Reference Ltd., London, 2001), pp. 1–7Google Scholar
- 13.R.H. Reed, G.M. Gadd, in Heavy Metal Tolerance in Plants: Evolutionary Aspects, ed. by A.J. Shaw. (CRC Press, Boca Raton, FL. 1990), pp. 105–118Google Scholar
- 14.de Filippis L.F., Pallaghy C.K.: Z. Pflanzenphysiol. 78, 314 (1992)Google Scholar
- 19.Sikora J., Żurawski J., Rutkowska J., Poniedziałek B., Wiktorowicz K., Dudkowiak A.: Ochr. Środ. Zas. Nat. 41, 293 (2009)Google Scholar
- 23.Rippka R., Deruelles J., Waterbury J.B., Herdman M., Stainer R.Y.: J. Gen. Microbiol. 111, 1 (1979)Google Scholar
- 30.Cotterill R.M.J.: Biophysics. Wiley, Chichester (2002)Google Scholar