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International Journal of Primatology

, Volume 29, Issue 1, pp 245–263 | Cite as

Examination of the Taxonomy and Diversification of Leontopithecus using the Mitochondrial Control Region

  • Beatriz M. Perez-SweeneyEmail author
  • Claudio Valladares-Padua
  • Cristiana Saddy Martins
  • Juan Carlos Morales
  • Don J. MelnickEmail author
Article

Abstract

Leontopithecus comprises 4 taxa: black lion tamarins (L. chrysopygus), golden lion tamarins (L. rosalia), black-faced lion tamarins (L. caissara), and golden-headed lion tamarins (L. chrysomelas). Endemic to the Atlantic Forest of Brazil, they are endangered (Appendix I, CITES; IUCN Critically Endangered: Leontopithecus chrysopygus, L. caissara; IUCN Endangered: L. rosalia, L. chrysomelas). The 4 taxa are differentiated morphologically and geographically and occupy different habitat types. However, it is not clear if all of them are separate species, particularly Leontopithecus caissara, or how they are related to one another evolutionarily. Therefore, we investigated lion tamarin differentiation and radiation. We sequenced the mtDNA control region and performed phylogenetic analyses, population aggregation analyses, and Mantel tests for geographic/genetic correlation. Mitochondrial genetic data suggest 3 distinct lion tamarin clades (Leontopithecus chrysomelas; L. caissara; and L. chrysopygus/L. rosalia). Phylogenetic analysis also supports: 1) the basal lion tamarin is Leontopithecus chrysomelas, and not L. chrysopygus, 2) L. caissara is not subspecific to L. chrysopygus, and 3) Quaternary forest refugia may have shaped lion tamarin diversification via a pattern that does not follow the theory of metachromism. Even though mitochondrial genetic analyses do not unequivocally support the 4 lion tamarins as separate species, one should consider the 4 lion tamarins, with equal conservation priority based on the combination of morphological, genetic, and habitat differentiation. Each of them is an extremely valuable flagship species that focuses attention on the diminishing, highly endemic Atlantic Forest of Brazil.

Keywords

Leontopithecus mitochondria phylogenetic refugia taxonomy 

Notes

Acknowledgments

The U.S. National Science Foundation (Award No. 9814257 to BMP-S, DJM), Primate Conservation, Inc. (BMP-S, DJM), Sheldon Sheps Foundation (Department of Anthropology, Columbia University to BMP-S), the Center for Environmental Research Conservation (CERC) at Columbia University, the IPÊ –(Institute for Ecological Research in Brazil), the New York Consortium for Evolutionary Primatology (BMP-S), and an NIH minority supplement (award HL61960–06 to John Ho for support of BMP-S) provided funding for the research. We completed sample collection and handling in compliance with the Animal Care and Use committee at Columbia University. We transferred BLT samples with all required U.S. permits (U.S. Fish and Wildlife Service and New York State Wildlife Authority) and Brazilian permits (IBAMA and CNPq). We thank Todd Disotell of New York University for his invaluable suggestions on data analysis. We thank Laury Cullen, Patricia Medici, Alcides Pissinati, and Fabiana Prado and the excellent field assistants at the IPÊ for their logistical and moral support during field collection.

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Beatriz M. Perez-Sweeney
    • 1
    Email author
  • Claudio Valladares-Padua
    • 2
  • Cristiana Saddy Martins
    • 2
  • Juan Carlos Morales
    • 3
  • Don J. Melnick
    • 4
    Email author
  1. 1.Weill Medical College of Cornell UniversityNew YorkUSA
  2. 2.IPÊInstituto de Pesquisas EcologicasNazare PaulistaBrazil
  3. 3.Division of Environmental Biology, Systematic Biology and Biodiversity InventoriesNational Science FoundationArlingtonUSA
  4. 4.Department of Ecology, Evolution, and Environmental BiologyColumbia UniversityNew YorkUSA

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