Advertisement

Inflammation

pp 1–10 | Cite as

MicroRNA-486-5p Promotes Acute Lung Injury via Inducing Inflammation and Apoptosis by Targeting OTUD7B

  • Qiang Luo
  • Jun Zhu
  • Qian Zhang
  • Jie Xie
  • Chengla Yi
  • Tianyu LiEmail author
Original Article
  • 26 Downloads

Abstract

The aim of this article is to study the effect of miR-486-5p in acute lung injury (ALI). MiR-486-5p expression in peripheral blood was determined in ALI patients and healthy volunteers by qRT-PCR. ALI mouse model were reproduced by LPS treatment, and miR-486-5p NC and miRNA-486 inhibitors were injected through trachea. ALI patients’ peripheral blood and LPS-induced acute lung injury in mice had significantly higher miR-486-5p levels than control subjects. Inhibition of miR-486-5p by injection with antagomiR-486-5p markedly reduced LPS-induced lung inflammation. Moreover, knockdown of miR-486-5p can reduce protects A549 cell against LPS-induced injury and its corresponding inflammatory response. In addition, Mechanistic analysis indicated that miR-486-5p on the occurrence of ALI is related to the inhibition of OTUD7B activity, which induces the downregulation of inflammatory in ALI. Our results identified miR-486-5p independently associated with ALI. miR-486-5p can mediate the formation of ALI by promoting inflammation.

KEY WORDS

Acute lung injury miR-486-5p OTUD7B Inflammation LPS Apoptosis 

Notes

Compliance with Ethical Standards

All mouse experiments were approved by research committee of Huazhong University of Science and Technology (Wuhan, China). This study was approved by the Tongji Hospital and written informed consent was obtained from all patients.

Supplementary material

10753_2020_1183_MOESM1_ESM.xls (26 kb)
ESM 1 (XLS 25 kb)
10753_2020_1183_MOESM2_ESM.xls (26 kb)
ESM 2 (XLS 26 kb)

References

  1. 1.
    Ameis, D., N. Khoshgoo, B.M. Iwasiow, P. Snarr, and R. Keijzer. 2017. MicroRNAs in lung development and disease. Paediatric Respiratory Reviews 22: 38–43.  https://doi.org/10.1016/j.prrv.2016.12.002.CrossRefPubMedGoogle Scholar
  2. 2.
    Bachofen, M., and E.R. Weibel. 1982. Structural alterations of lung parenchyma in the adult respiratory distress syndrome. Clinics in Chest Medicine 3 (1): 35–56.PubMedGoogle Scholar
  3. 3.
    Bartel, D.P. 2004. MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell 116 (2): 281–297.  https://doi.org/10.1016/s0092-8674(04)00045-5.CrossRefPubMedGoogle Scholar
  4. 4.
    Cai, Z.G., S.M. Zhang, Y. Zhang, Y.Y. Zhou, H.B. Wu, and X.P. Xu. 2012. MicroRNAs are dynamically regulated and play an important role in LPS-induced lung injury. Canadian Journal of Physiology and Pharmacology 90 (1): 37–43.  https://doi.org/10.1139/y11-095.CrossRefPubMedGoogle Scholar
  5. 5.
    Cao, D.D., L. Li, and W.Y. Chan. 2016. MicroRNAs: Key regulators in the central nervous system and their implication in neurological diseases. International Journal of Molecular Sciences 17 (6).  https://doi.org/10.3390/ijms17060842.CrossRefGoogle Scholar
  6. 6.
    Chen, H., C. Bai, and X. Wang. 2010. The value of the lipopolysaccharide-induced acute lung injury model in respiratory medicine. Expert Review of Respiratory Medicine 4 (6): 773–783.  https://doi.org/10.1586/ers.10.71.CrossRefPubMedGoogle Scholar
  7. 7.
    Chi, G., M. Wei, X. Xie, L.W. Soromou, F. Liu, and S. Zhao. 2013. Suppression of MAPK and NF-kappaB pathways by limonene contributes to attenuation of lipopolysaccharide-induced inflammatory responses in acute lung injury. Inflammation 36 (2): 501–511.  https://doi.org/10.1007/s10753-012-9571-1.CrossRefPubMedGoogle Scholar
  8. 8.
    Essandoh, K., Y. Li, J. Huo, and G.C. Fan. 2016. MiRNA-mediated macrophage polarization and its potential role in the regulation of inflammatory response. Shock 46 (2): 122–131.  https://doi.org/10.1097/shk.0000000000000604.CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Fein, A., R.F. Grossman, J.G. Jones, E. Overland, L. Pitts, J.F. Murray, and N.C. Staub. 1979. The value of edema fluid protein measurement in patients with pulmonary edema. The American Journal of Medicine 67 (1): 32–38.  https://doi.org/10.1016/0002-9343(79)90066-4.CrossRefPubMedGoogle Scholar
  10. 10.
    Fleshner, M., and C.R. Crane. 2017. Exosomes, DAMPs and miRNA: Features of stress physiology and immune homeostasis. Trends in Immunology 38 (10): 768–776.  https://doi.org/10.1016/j.it.2017.08.002.CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Galani, V., E. Tatsaki, M. Bai, P. Kitsoulis, M. Lekka, G. Nakos, and P. Kanavaros. 2010. The role of apoptosis in the pathophysiology of acute respiratory distress syndrome (ARDS): An up-to-date cell-specific review. Pathology, Research and Practice 206 (3): 145–150.  https://doi.org/10.1016/j.prp.2009.12.002.CrossRefPubMedGoogle Scholar
  12. 12.
    Gao, Z.J., W.D. Yuan, J.Q. Yuan, K. Yuan, and Y. Wang. 2018. miR-486-5p functions as an oncogene by targeting PTEN in non-small cell lung cancer. Pathology, Research and Practice 214 (5): 700–705.  https://doi.org/10.1016/j.prp.2018.03.013.CrossRefPubMedGoogle Scholar
  13. 13.
    Goolaerts, A., J. Roux, M.T. Ganter, V. Shlyonsky, A. Chraibi, R. Stephane, F. Mies, et al. 2010. Serotonin decreases alveolar epithelial fluid transport via a direct inhibition of the epithelial sodium channel. American Journal of Respiratory Cell and Molecular Biology 43 (1): 99–108.  https://doi.org/10.1165/rcmb.2008-0472OC.CrossRefPubMedGoogle Scholar
  14. 14.
    Gouda, M.M., S.B. Shaikh, and Y.P. Bhandary. 2018. Inflammatory and fibrinolytic system in acute respiratory distress syndrome. Lung 196 (5): 609–616.  https://doi.org/10.1007/s00408-018-0150-6.CrossRefPubMedGoogle Scholar
  15. 15.
    Hellwig, S.M., H.F. van Oirschot, W.L. Hazenbos, A.B. van Spriel, F.R. Mooi, and J.G. van De Winkel. 2001. Targeting to Fcgamma receptors, but not CR3 (CD11b/CD18), increases clearance of Bordetella pertussis. The Journal of Infectious Diseases 183 (6): 871–879.  https://doi.org/10.1086/319266.CrossRefPubMedGoogle Scholar
  16. 16.
    Huang, W. 2017. MicroRNAs: Biomarkers, diagnostics, and therapeutics. Methods in Molecular Biology 1617: 57–67.  https://doi.org/10.1007/978-1-4939-7046-9_4.CrossRefPubMedGoogle Scholar
  17. 17.
    Ioan-Facsinay, A., S.J. de Kimpe, S.M. Hellwig, P.L. van Lent, F.M. Hofhuis, H.H. van Ojik, C. Sedlik, et al. 2002. FcgammaRI (CD64) contributes substantially to severity of arthritis, hypersensitivity responses, and protection from bacterial infection. Immunity 16 (3): 391–402.  https://doi.org/10.1016/s1074-7613(02)00294-7.CrossRefPubMedGoogle Scholar
  18. 18.
    Jiang, K., T. Zhang, N. Yin, X. Ma, G. Zhao, H. Wu, C. Qiu, and G. Deng. 2017. Geraniol alleviates LPS-induced acute lung injury in mice via inhibiting inflammation and apoptosis. Oncotarget 8 (41): 71038–71053.  https://doi.org/10.18632/oncotarget.20298.CrossRefPubMedPubMedCentralGoogle Scholar
  19. 19.
    Lan, C.C., C.K. Peng, S.E. Tang, H.J. Lin, S.S. Yang, C.P. Wu, and K.L. Huang. 2017. Inhibition of Na-K-cl cotransporter isoform 1 reduces lung injury induced by ischemia-reperfusion. The Journal of Thoracic and Cardiovascular Surgery 153 (1): 206–215.  https://doi.org/10.1016/j.jtcvs.2016.09.068.CrossRefPubMedGoogle Scholar
  20. 20.
    Li, Z., W. Jiang, G. Wu, X. Ju, Y. Wang, and W. Liu. 2018. miR-16 inhibits hyperoxia-induced cell apoptosis in human alveolar epithelial cells. Molecular Medicine Reports 17 (4): 5950–5957.  https://doi.org/10.3892/mmr.2018.8636.CrossRefPubMedGoogle Scholar
  21. 21.
    Liu, B., X. Lu, C. Qi, S. Zheng, M. Zhou, J. Wang, and W. Yin. 2014. KGFR promotes Na+ channel expression in a rat acute lung injury model. African Health Sciences 14 (3): 648–656.  https://doi.org/10.4314/ahs.v14i3.21.CrossRefPubMedPubMedCentralGoogle Scholar
  22. 22.
    Liu, C., M. Li, Y. Hu, N. Shi, H. Yu, H. Liu, and H. Lian. 2016. miR-486-5p attenuates tumor growth and lymphangiogenesis by targeting neuropilin-2 in colorectal carcinoma. OncoTargets and Therapy 9: 2865–2871.  https://doi.org/10.2147/ott.S103460.CrossRefPubMedPubMedCentralGoogle Scholar
  23. 23.
    Ma, X., J. Wei, L. Zhang, D. Deng, L. Liu, X. Mei, X. He, and J. Tian. 2016. miR-486-5p inhibits cell growth of papillary thyroid carcinoma by targeting fibrillin-1. Biomedicine & Pharmacotherapy 80: 220–226.  https://doi.org/10.1016/j.biopha.2016.03.020.CrossRefGoogle Scholar
  24. 24.
    Matthay, M.A., L.B. Ware, and G.A. Zimmerman. 2012. The acute respiratory distress syndrome. The Journal of Clinical Investigation 122 (8): 2731–2740.  https://doi.org/10.1172/jci60331.CrossRefPubMedPubMedCentralGoogle Scholar
  25. 25.
    Matthay, M.A., R.L. Zemans, G.A. Zimmerman, Y.M. Arabi, J.R. Beitler, A. Mercat, M. Herridge, A.G. Randolph, and C.S. Calfee. 2019. Acute respiratory distress syndrome. Nature Reviews. Disease Primers 5 (1): 18.  https://doi.org/10.1038/s41572-019-0069-0.CrossRefPubMedPubMedCentralGoogle Scholar
  26. 26.
    Mizuno, K., H. Mataki, N. Seki, T. Kumamoto, K. Kamikawaji, and H. Inoue. 2017. MicroRNAs in non-small cell lung cancer and idiopathic pulmonary fibrosis. Journal of Human Genetics 62 (1): 57–65.  https://doi.org/10.1038/jhg.2016.98.CrossRefPubMedGoogle Scholar
  27. 27.
    Moles, R. 2017. MicroRNAs-based therapy: A novel and promising strategy for Cancer treatment. Microrna 6 (2): 102–109.  https://doi.org/10.2174/2211536606666170710183039.CrossRefPubMedGoogle Scholar
  28. 28.
    Pham, T., and G.D. Rubenfeld. 2017. Fifty years of research in ARDS. The epidemiology of acute respiratory distress syndrome. A 50th birthday review. American Journal of Respiratory and Critical Care Medicine 195 (7): 860–870.  https://doi.org/10.1164/rccm.201609-1773CP.CrossRefPubMedGoogle Scholar
  29. 29.
    Saba, R., S. Gushue, R.L. Huzarewich, K. Manguiat, S. Medina, C. Robertson, and S.A. Booth. 2012. MicroRNA 146a (miR-146a) is over-expressed during prion disease and modulates the innate immune response and the microglial activation state. PLoS One 7 (2): e30832.  https://doi.org/10.1371/journal.pone.0030832.CrossRefPubMedPubMedCentralGoogle Scholar
  30. 30.
    Vadasz, I., S. Raviv, and J.I. Sznajder. 2007. Alveolar epithelium and Na,K-ATPase in acute lung injury. Intensive Care Medicine 33 (7): 1243–1251.  https://doi.org/10.1007/s00134-007-0661-8.CrossRefPubMedGoogle Scholar
  31. 31.
    van Lent, P., K.C. Nabbe, P. Boross, A.B. Blom, J. Roth, A. Holthuysen, A. Sloetjes, S. Verbeek, and W. van den Berg. 2003. The inhibitory receptor FcgammaRII reduces joint inflammation and destruction in experimental immune complex-mediated arthritides not only by inhibition of FcgammaRI/III but also by efficient clearance and endocytosis of immune complexes. The American Journal of Pathology 163 (5): 1839–1848.  https://doi.org/10.1016/s0002-9440(10)63543-2.CrossRefPubMedPubMedCentralGoogle Scholar
  32. 32.
    Vlaar, A.P., and N.P. Juffermans. 2013. Transfusion-related acute lung injury: A clinical review. Lancet 382 (9896): 984–994.  https://doi.org/10.1016/s0140-6736(12)62197-7.CrossRefPubMedGoogle Scholar
  33. 33.
    Xie, T., J. Liang, N. Liu, Q. Wang, Y. Li, P.W. Noble, and D. Jiang. 2012. MicroRNA-127 inhibits lung inflammation by targeting IgG Fcgamma receptor I. Journal of Immunology 188 (5): 2437–2444.  https://doi.org/10.4049/jimmunol.1101070.CrossRefGoogle Scholar
  34. 34.
    Ying, H., Y. Kang, H. Zhang, D. Zhao, J. Xia, Z. Lu, H. Wang, F. Xu, and L. Shi. 2015. MiR-127 modulates macrophage polarization and promotes lung inflammation and injury by activating the JNK pathway. Journal of Immunology 194 (3): 1239–1251.  https://doi.org/10.4049/jimmunol.1402088.CrossRefGoogle Scholar
  35. 35.
    Zeng, Z., H. Gong, Y. Li, K. Jie, C. Ding, Q. Shao, F. Liu, Y. Zhan, C. Nie, W. Zhu, and K. Qian. 2013. Upregulation of miR-146a contributes to the suppression of inflammatory responses in LPS-induced acute lung injury. Experimental Lung Research 39 (7): 275–282.  https://doi.org/10.3109/01902148.2013.808285.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Trauma Center/Department of Emergency and Traumatic SurgeryTongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyWuhanChina
  2. 2.Department of AnesthesiologyThe Fourth Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and TechnologyWuhanChina

Personalised recommendations