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Periodontal Disease Impairs Muscle Recovery by Modulating the Recruitment of Leukocytes

  • Bárbara Capitanio de SouzaEmail author
  • Bibiana Franzen Matte
  • André Luiz Lopes
  • Bruno Costa Teixeira
  • Marcelo Lazzaron Lamers
Original Article


The purpose of this study is to analyze the impact of periodontal disease (PD) associated with physical exercise on inflammatory mediators and muscle repair. Twenty-four Wistar rats were divided into four groups: control (SH), healthy trained (TH), sedentary with PD (SP), and trained with PD (TP). PD was induced in groups SP and TP while the trained groups performed treadmill exercises for 8 weeks. For the analysis of IL-6, IL-10, TNF-α, and leukocyte count, we collected blood samples. Cryolesions were induced in the tibialis anterior and gastrocnemius, which were analyzed for morphological changes. The presence of PD modified leukocyte counts, while exercise showed an additive role. PD increased levels of IL-6, IL-10, and TNF-α, and physical exercise changed only values of IL-10. The association between physical exercise and PD was responsible for an increased concentration of leukocytes in the region of the inflammation. Serum levels of inflammatory markers were modified by PD and, when combined with exercise, may negatively modulate inflammation. The association between PD and physical exercise showed the most significant changes in the number of inflammatory cells and may negatively influence the process of muscle repair.


physical education and training athletic performance inflammation muscular diseases oral health 


Compliance with Ethical Standards

All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

10753_2019_1128_MOESM1_ESM.jpg (311 kb)
Supplemental Fig. 1 Replacing the thymic parenchyma by adipose tissue. V=Pp/Ptx100% formula was used to calculate the volumetric density of adipose tissue present in the thymic parenchyma, where Pp is the number of points Pt on fat cells and the number of points of the test system (A). SH: control, SP: sedentary with periodontal disease, TH: trained without periodontal disease, TP: trained with periodontal disease. Hematoxylin-eosin staining. 200X magnification (JPG 310 kb)
10753_2019_1128_MOESM2_ESM.jpg (148 kb)
Supplemental Fig. 2 Test system for analysis of inflammatory intensity. V=Pp/Ptx100% formula was used to calculate the volumetric density of inflammatory cells, where Pp is the number of points on Pt inflammatory cells and the number of points of the test system. Hematoxylin-eosin staining. 400X magnification (JPG 148 kb)
10753_2019_1128_MOESM3_ESM.docx (17 kb)
Supplementary Table S1 (DOCX 16 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019
corrected publication December/2019

Authors and Affiliations

  1. 1.Dentistry SchoolFederal University of Rio Grande do Sul (UFRGS)Porto AlegreBrazil
  2. 2.Laboratory of Exercise Research (LAPEX), College of Physical Education (ESEF)Federal University of Rio Grande do Sul (UFRGS)Porto AlegreBrazil
  3. 3.Department of Morphological SciencesFederal University of Rio Grande do Sul (UFRGS)Porto AlegreBrazil

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