Sudachitin Inhibits Matrix Metalloproteinase-1 and -3 Production in Tumor Necrosis Factor-α-Stimulated Human Periodontal Ligament Cells

  • Yoshitaka HosokawaEmail author
  • Ikuko Hosokawa
  • Kazumi Ozaki
  • Takashi Matsuo


Sudachitin, a polymethoxylated flavonoid found in the skin of Citrus sudachi, is a biologically active substance. The aim of this study was to examine whether sudachitin could be used to inhibit the expression of matrix metalloproteinase (MMP)-1 and MMP-3, which are involved in the destruction of periodontal tissues in periodontal lesions, in tumor necrosis factor (TNF)-α-stimulated human periodontal ligament cells (HPDLC). Sudachitin suppressed TNF-α-induced MMP-1 and MMP-3 production in HPDLC. On the other hand, it enhanced tissue inhibitor of metalloproteinase (TIMP)-1 expression. The level of Akt phosphorylation in the TNF-α-stimulated HPDLC was decreased by sudachitin treatment. Moreover, an Akt inhibitor reduced MMP-1 and MMP-3 production and increased TIMP-1 production. These findings indicate that sudachitin reduces MMP-1 and MMP-3 production in TNF-α-stimulated HPDLC by inhibiting the Akt pathway.


sudachitin MMP Akt periodontal ligament cells 


Funding Information

This study was supported by JSPS KAKENHI (grant numbers: 16K11834 and 18K09600).

Compliance with Ethical Standards

Conflicts of Interest

The authors declare that they have no conflicts of interest.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Yoshitaka Hosokawa
    • 1
    Email author
  • Ikuko Hosokawa
    • 1
  • Kazumi Ozaki
    • 2
  • Takashi Matsuo
    • 1
  1. 1.Department of Conservative Dentistry, Institute of Biomedical SciencesTokushima University Graduate SchoolTokushimaJapan
  2. 2.Department of Oral Health Care Promotion, Institute of Biomedical SciencesTokushima University Graduate SchoolTokushimaJapan

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