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Anti-inflammatory Activity of Methanolic Extract and an Alkaloid from Palicourea crocea (Sw.) Roem and Schult

  • Anelise Samara Nazari FormagioEmail author
  • Pedro Cruz de Oliveira Junior
  • Carla Roberta Ferreira Volobuff
  • Candida Aparecida Leite Kassuya
  • Diego Cegobia Ferreira
  • Claudia Andrea Lima Cardoso
  • Maria Helena Sarragiotto
  • Zefa Valdevina Pereira


Palicourea crocea (Sw.) Roem. and Schult., “douradinha,” are used by treat inflammation (edema). Croceaine A (PC-1) was isolated from P. crocea (MEPC) leaves and studied for its antioxidant and anti-inflammatory activity, as well as concentrations of constituents and acute toxicity. The phenols and polyphenolics compounds and HPLC/DAD were determined. The antioxidant activity were evaluated for DPPH, ABTS, and MDA. MEPC (300, 100, and 300 mg/kg) and PC-1 (10 and 30 mg/kg) were tested for anti-inflammatory effects in paw edema, pleurisy, cold sensitivity, and mechanical hyperalgesia. Acute toxicity is also described. MEPC contained high concentrations of phenolic and flavonoid compounds (≤ 800.35 mg/g), as well as caffeic acid, ferulic acid, rutin, and quercetin, revealed by HPLC-DAD analysis. MEPC displayed antioxidant activity against ABTS radicals (IC50 = 68.5 μg/mL) and MDA (74%). MEPC and alkaloid PC-1 demonstrated an anti-edematogenic effect in Cg-induced paw edema in 2 and 4 h, and also significantly reduced mechanical hyperalgesia, cold response to acetone in mice, at 3 and 4 h after injection, as well as leukocyte migrationin the pleurisy model. No toxicity was detected by MEPC. For the first time, P. crocea was evaluated for its antioxidant, systemic anti-inflammatory, and anti-hyperalgesic activities.


antioxidant anti-hyperalgesic croceaine A douradina edema pleurisy 


Funding Information

We are grateful to CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil, 2764/2011) and UFGD (Universidade Federal da Grande Dourados) for financial support and fellowships.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Ethical Approval

This work was found to comply with the ethical concepts required by the National Council for Animal Experiments Control (CONCEA), with current legislation and other provisions of the ethics investigations that directly involve the use of animals and all protocols and procedures were approved according to the Ethics Committee on Animal Use the Federal University of Grande Dourados, Mato Grosso do Sul, Brazil (CEUA/UFGD) with authorization number 17/2017.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Anelise Samara Nazari Formagio
    • 1
    • 2
    Email author
  • Pedro Cruz de Oliveira Junior
    • 1
  • Carla Roberta Ferreira Volobuff
    • 2
  • Candida Aparecida Leite Kassuya
    • 2
  • Diego Cegobia Ferreira
    • 3
  • Claudia Andrea Lima Cardoso
    • 3
  • Maria Helena Sarragiotto
    • 4
  • Zefa Valdevina Pereira
    • 1
  1. 1.Faculty of Biological and Environmental SciencesFederal University of Grande Dourados UFGDDouradosBrazil
  2. 2.Faculty of Health SciencesFederal University of Grande Dourados UFGDDouradosBrazil
  3. 3.ChemistryState University of Mato Grosso do Sul UEMSDouradosBrazil
  4. 4.ChemistryState University of Maringá UEMMaringáBrazil

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