Electroacupuncture Pretreatment Attenuates Inflammatory Lung Injury After Cardiopulmonary Bypass by Suppressing NLRP3 Inflammasome Activation in Rats
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Cardiopulmonary bypass (CPB) can induce inflammatory lung injury, which is a common complication during cardiac surgery. Nod-like receptor family, pyrin domain-containing 3 (NLRP3) inflammasome-induced inflammation plays a crucial role in lung injury after CPB. Previous studies have shown that electroacupuncture (EA) has potential anti-inflammatory activity. However, the role of EA in CPB is poorly understood. The aim of this study was to determine whether EA was associated with CPB-induced inflammatory lung injury. In the present study, rats were treated with EA for 5 days before CPB. Two hours after CPB, the lung tissue, serum, and bronchoalveolar lavage fluid (BALF) were prepared for assessment. Our results showed that the expression of NLRP3 in the lung tissue increased significantly after CPB. The EA pretreatment suppressed NLRP3 inflammasome activation, reduced lung edema, and inhibited IL-1β release into the serum and BALF after CPB. Our findings suggest that EA pretreatment attenuates inflammatory lung injury after CPB by suppressing NLRP3 inflammasome activation.
KEY WORDSElectroacupuncture Inflammatory lung injury Cardiopulmonary bypass NLRP3 IL-1β Caspase-1
We thank the staff of the Department of Anesthesiology Laboratory at the Shanghai Ren Ji Hospital for their assistance.
WY conceived and designed the study. DH, MC, ZW, and LH performed the experiments and analyzed the data. DH and MC wrote the manuscript. WY reviewed and edited the manuscript. All the authors read and approved the manuscript.
This study was funded by the Wuxi Municipal Commission Fund of Health and Family Planning [MS201713].
Compliance with Ethical Standards
All animal experiments were approved by the Animal Care and Use Committee of Shanghai Jiao Tong University, School of Medicine. All the animal procedures conformed to the National Institutes of Health Guide for the Care and Use of Laboratory Animals (NIH Publications No. 8023, revised 1978).
The authors declare that they have no competing interests.
Informed consent was obtained from all individual participants included in the study.
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