, Volume 42, Issue 2, pp 721–730 | Cite as

Breast Implant Texturization Does Not Affect the Crosstalk Between MSC and ALCL Cells

  • Monia OrcianiEmail author
  • Miriam Caffarini
  • Matteo Torresetti
  • Anna Campanati
  • Piercamillo Parodi
  • Giovanni Di Benedetto
  • Roberto Di Primio


In the last decade, there has been a growing interest about the possible association between anaplastic large cell lymphoma (ALCL) and breast implants (BIA-ALCL). Many variables, such as breast implants texturization, have been investigated. Breast implants often lead to the formation of a periprosthetic capsule, characterized by inflammation. The presence of the inflamed capsule has been found in the majority of patients with BIA-ALCL. Inflammation may be sustained or counteracted by mesenchymal stem cells (MSCs) by the secretion of pro- or anti-inflammatory cytokines. MSCs were isolated from three capsules surrounding micro-textured (micro-MSCs) and from three capsules surrounding macro-textured (macro-MSCs) implants; after characterization, MSCs were co-cultured with KI-JK cells (a cell line derived from the cutaneous form of ALCL). The secretion of cytokines related to inflammation, the proliferation rate, and the expression of genes referred to pro-tumoral mechanisms were evaluated. Co-cultures of KI-JK cells with micro- or macro-MSCs gave the same results about the secretion of cytokines (increase of IL10, G-CSF, and TGF-β1 and decrease of IL4, IL5, IL12, IL13, IL17A, IFN-γ (p < 0.05) with respect to mock sample), expression of selected genes (increase for ACVR1, VEGF, TGF-βR2, CXCL12, and MKi67 (p < 0.05) with respect to control sample), and the proliferation rate (no variation between mock and co-cultured samples). Our results suggest that MSCs derived from capsules surrounding micro- and macro-textured implants display the same effects on the ALCL cells.


breast implants mesenchymal stem cells inflammation texturization ALCL 


Compliance with Ethical Standards

This study was approved by the Università Politecnica delle Marche Ethical Commitee and was conducted in accordance with the Declaration of Helsinki.

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

10753_2018_930_Fig5_ESM.png (913 kb)
Supplementary Fig. 1

Flow cytometry results of micro-MSCs cells (a) and macro-MSCs (b). Representative FACScan analyses of cell-surface antigen expression, as indicated. Solid gray histograms refer to the negative control (IgG1 isotype control-FITC labeled). No significative differences were noted among the six cultures of MSCs. (PNG 912 kb)

10753_2018_930_MOESM1_ESM.tif (229 kb)
High resolution image (TIF 228 kb)
10753_2018_930_Fig6_ESM.png (3.5 mb)
Supplementary Fig. 2

Mesenchymal differentiation from micro-MSCs (a-b-c-d) and macro-MSCs (e-f-g-h). Representative images of osteogenic differentiation assessment by ALP staining (a, e) and identification of CaPO4 crystals by von Kossa reaction (b, f); adipocyte differentiation confirmed by Oil red staining (c, g); chondrogenic differentiation demonstrated by positive acid mucopolisaccarid coloration by Safranin-O (d, h). No differences were noted among micro- and macro-MSCs. Scale bar 50um (PNG 3615 kb)

10753_2018_930_MOESM2_ESM.tif (879 kb)
High resolution image (TIF 879 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Clinical and Molecular Sciences- HistologyUniversità Politecnica delle MarcheAnconaItaly
  2. 2.Department of Experimental and Clinical Medicine – Clinic of Plastic and Reconstructive SurgeryUniversità Politecnica delle MarcheAnconaItaly
  3. 3.Department of Clinical and Molecular Sciences- Clinic of DermatologyUniversità Politecnica delle MarcheAnconaItaly
  4. 4.Clinic of Plastic and Reconstructive Surgery of UdineUniversity of UdineUdineItaly

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