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Inflammation

pp 1–10 | Cite as

Effects of Human Interleukin-10 on Ventilator-Associated Lung Injury in Rats

  • Jinzhuan Chen
  • Jianqing Lin
  • Huiqin Luo
  • Minjie Li
ORIGINAL ARTICLE
  • 77 Downloads

Abstract

Ventilator-induced lung injury (VILI) is one of the most serious complications of mechanical ventilation (MV) and can increase the mortality of patients with acute respiratory distress syndrome (ARDS). This work aimed to test the hypothesis that the anti-inflammatory properties of human interleukin-10 (hIL-10) can reduce VILI. Thirty-six healthy male Sprague-Dawley rats were randomly assigned into three groups (n = 12) as follows: a control group, a VILI group, and a hIL-10 group. Lung function was evaluated by oxygenation index and pulmonary edema, and morphological changes associated with lung injury were assessed by HE staining and quantitative histological lung injury score. Malondialdehyde (MDA) and Superoxide dismutase (SOD) were measured, and the levels of various inflammatory cytokines were assessed in BALF and plasma. The oxygenation index in the VILI group decreased significantly relative to the control group and improved substantially in the hIL-10 group (P < 0.01). Compared to the control group, MDA production was stimulated (P < 0.01), and SOD activity rapidly declined (P < 0.01) in the VILI group. After hIL-10, MDA content was lower than that seen in the VILI group (P < 0.01), and SOD activity was enhanced (P < 0.01). The VILI group had the highest cytokine levels, compared to either the hIL-10 group or the control group (P < 0.05). High tidal volume MV can induce VILI. hIL-10 may regulate the inflammatory response in the lung tissue, improve lung tissue oxygenation, and inhibit oxidative stress, therefore reducing VILI in rats. These experiments reveal a potential new treatment option for VILI.

Key Words

human interleukin-10 ventilator-associated lung injury mechanical ventilation 

Notes

FUNDING INFORMATION

This work was supported by the Science and Technology Project of Education Department in Fujian Province (Project No. JK2014017), medical innovation program of Fujian Provincial Health Bureau (2016-CX-43).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Jinzhuan Chen
    • 1
  • Jianqing Lin
    • 1
  • Huiqin Luo
    • 2
  • Minjie Li
    • 1
  1. 1.Department of Anesthesiologythe First Hospital of Fujian Medical UniversityFuzhouChina
  2. 2.Department of Anesthesiologythe Affiliated People’s Hospital of Fujian University of Traditional Chinese MedicineFuzhouChina

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