Role of Thymoquinone in Cardiac Damage Caused by Sepsis from BALB/c Mice
Sepsis is a major health complication causing patient mortality and increased healthcare costs. Cardiac dysfunction, an important consequence of sepsis, affects mortality. We previously reported that thymoquinone (TQ) protected against hyperlipidemia and doxorubicin-induced cardiac damage. This study investigated the possible protective effects of TQ against cardiac damage in septic BALB/c mice. Eight-week-old male BALB/c mice were divided into four groups: control, TQ, cecal ligation and puncture (CLP), and TQ + CLP. CLP was performed after 2-week TQ gavage. After 48 h, we measured the histopathological alterations of the cardiac tissue and the plasma levels of troponin-T (cTnT) and ATP. We evaluated autophagy (p62 and beclin 1), pyroptosis (NLRP3, caspase-1, interleukin [IL]-1β, and IL-18) at the gene and protein levels and IL-6 and tumor necrosis factor-α (TNF-α) at the gene level. Our results demonstrated that TQ administration significantly reduced intestinal histological alterations. TQ inhibited plasma cTnT levels; improved ATP; significantly inhibited p62, NLRP3, caspase-1, IL-1β, IL-18, IL-6, TNF-α, and MCP-1expressions; and increased beclin 1 and IL-10 level. The phosphatidylinositide 3-kinase level was significantly decreased in the TQ + CLP group versus the CLP group. These results suggest that TQ effectively modulates autophagy, pyroptosis, and pro-inflammatory, making it important in the treatment of sepsis-induced cardiac damage.
KEY WORDSsepsis thymoquinone cardiac damage pyroptosis BALB/c mice
Zuowei Pei designed this study; Hongyang Liu, Guang Yang, and Yue Zhao helped in performing experiments; Zuowei Pei and Yan Sun analyzed data and interpreted the results of experiments; Lipeng Guo and Ying Zhang prepared figures; Hongyang Liu drafted the manuscript. All authors read and approved the final manuscript.
This work was finally supported by the Posterdoctor Foundation of Liaoning Province, China (No. 194008).
Compliance with Ethical Standards
Conflicts of Interest
The authors declare that they have no conflict of interest.
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