, Volume 42, Issue 1, pp 264–275 | Cite as

TanshinoneIIA Alleviates Inflammatory Response and Directs Macrophage Polarization in Lipopolysaccharide-Stimulated RAW264.7 Cells

  • Shan Gao
  • Yili Wang
  • Dan Li
  • Yuying Guo
  • Meifeng Zhu
  • Shixin Xu
  • Jingyuan Mao
  • Guanwei FanEmail author


TanshinoneIIA (TanIIA) has been demonstrated to possess numerous biological effects. However, the specific effect of TanIIA on macrophage polarization has not been reported. In this study, it was revealed that TanIIA might play a pivotal role in macrophage polarization. As our results indicated, cell morphology was changed in RAW264.7 cells which were treated with LPS or LPS/TanIIA (0.1 μM, 1 μM, 10 μM). Subsequently, pro-inflammatory cytokine TNF-α and anti-inflammatory cytokine IL-10 were measured by ELISA kits. Furthermore, TanIIA enhanced the expression of M2 macrophage markers (Arg1 and FIZZ1) and decreased the expression of markers associated with M1 macrophage polarization (iNOS and IL-1β). Increased expression of CD206 was also detected by flow cytometry in TanIIA-treated groups. Mechanistically, it was revealed that TanIIA modulated macrophage polarization by ameliorating mitochondrial function and regulating TLR4-HMGB1/CEBP-β pathway. In addition, increased expression of miR-155 was observed in RAW264.7 cells incubated with LPS and were effectively inhibited by TanIIA. Taken together, it was suggested that TanIIA inhibits inflammatory response and promotes macrophage polarization toward an M2 phenotype, which provides new evidence for the anti-inflammation activity of TanIIA.


TanIIA macrophage polarization cell elongation mitochondrial function TLR4-HMGB1/CEBP-β pathway 


Funding Information

This study was supported by the Tianjin Outstanding Youth Science Foundation (No. 17JCJQJC46200), the National Natural Science Foundation of China (No. 81774050), the National Key Basic Research Program of China (No. 2012CB518404), the Foundation of First Teaching Hospital of Tianjin University of Traditional Chinese Medicine (No. 201703), and the Tianjin Science and Technology Program: Tianjin TCM Clinical Medicine Research Center (No. 15ZXLCSY00020).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that there are no conflicts of interest.

Supplementary material

10753_2018_891_MOESM1_ESM.doc (722 kb)
ESM 1 (DOC 722 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Shan Gao
    • 1
  • Yili Wang
    • 2
  • Dan Li
    • 1
  • Yuying Guo
    • 1
  • Meifeng Zhu
    • 3
  • Shixin Xu
    • 1
  • Jingyuan Mao
    • 1
  • Guanwei Fan
    • 1
    Email author
  1. 1.Medical Experiment CenterFirst Teaching Hospital of Tianjin University of Traditional Chinese MedicineTianjinChina
  2. 2.Tianjin University of Traditional Chinese MedicineTianjinChina
  3. 3.State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials of Ministry of Education, College of Life ScienceNankai UniversityTianjinChina

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