, Volume 41, Issue 6, pp 2196–2205 | Cite as

Blockade of Aquaporin 4 Inhibits Irradiation-Induced Pulmonary Inflammation and Modulates Macrophage Polarization in Mice

  • Yuhui Li
  • Hongda Lu
  • Xiaojuan Lv
  • Qiu Tang
  • Wangxia Li
  • Hongfei Zhu
  • Yuan Long


To investigate the effects of aquaporin 4 (AQP4) inhibitor in irradiation-induced pulmonary inflammation in mice. A single dose of 75 Gy was delivered to the left lung of mice to induce radiation pneumonitis. For inhibition of AQP4, 200 mg/kg of TGN-020 was administered i.p. one time per 2 days post-irradiation. Blockade of AQP4 with TGN-020 resulted in the inhibition of inflammatory cell infiltration and the downregulation of inflammatory cytokines (IL-6, IL-17, and TGF-β), chemokines (MIP1a and MCP1), fibrosis-related (Col3al and Fn1), and M2 macrophage marker (Arg1) post-irradiation. Immunofluorescence staining indicated that there was significant fewer M2 macrophage infiltration in the irradiated lung tissues from mice treated with TGN-020. Additionally, depletion of macrophages with liposome clodronate resulted in alleviated lung injury induced by irradiation. Furthermore, adoptive transfer of M1 or M2 macrophages into clodronate-treated mice was performed. The results showed that the administration of M2 macrophages fully reversed the clodronate-induced beneficial effect on inflammation score, thickness, and fibrosis. However, transfer of M1 macrophages only impacted the inflammation score and thickness and did not affect lung fibrosis. AQP4 blockade alleviated the development and severity of irradiated lung damage. This was associated with attenuated infiltration of inflammatory cell, decreased production of pro-inflammatory cytokines, and inhibited activation of M2 macrophages.


AQP4 TGN-020 Cytokines Macrophages Alternatively activated M2 macrophages 


Funding Information

This work was supported by the Health and Family Planning Commission of Hubei Province of China (WJ2015CA002) and Scientific Research Project of Wuhan Health and Family Planning Commission (WX15D65).

Compliance with Ethical Standards

The data and maternal were available to the corresponding author.

Conflict of Interests

The authors declare that there is no conflict of interests.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Oncology, The Central Hospital of Wuhan, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  2. 2.HLA Typing LaboratoryBlood Center of WuhanWuhanChina
  3. 3.Department of Anesthesiology, Hubei Provincial Hospital of Traditional Chinese MedicalHubei Provincial Academy of Traditional Chinese MedicineWuhanChina
  4. 4.Department of Cardiaovascular Medicine, Wuhan Women and Children Medical Care Center, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina

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